Effects of Nd^(3+) and Sm^(3+) on the proliferation,differentiation and mineralization function of primary osteoblasts in vitro  

作　　者：ZHANG JinChao SHANG MiQin ZHANG DaWei LI YaPing SUN Jing CHEN Hang 

机构地区：[1]Chemical Biology Key Laboratory of Hebei Province, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China [2]Department of Natural Product Chemistry, Shenyang Pharmaceutical University, Shenyang 110016, China [3]B-Ultrasound Room, Affiliated Hospital of Hebei University, Baoding 071000, China [4]Department of Nephrology, Affiliated Hospital of Hebei University, Baoding 071000, China

出　　处：《Chinese Science Bulletin》2010年第23期2505-2511,共7页

基　　金：supported by the National Natural Science Foundation of China (20971034);the Natural Science Foundation of Hebei Province (B2009000161);Foundation for Key Program of Ministry of Education of China (208018);Returned Scholars of Hebei Province (207041);Natural Science Foundation of Hebei University

摘　　要：A series of experimental methods including 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide (MTT) test,alkaline phosphatase (ALP) activity measurement,Oil Red O stain and measurement,mineralized function expression and quantitive real time RT-PCR (qRT-PCR) were employed to assess the effect of Nd3+ and Sm3+ on the proliferation,differentiation and mineralization function of primary osteoblasts (OBs) in vitro at cell and molecular levels.The experimental results suggest that concentration,culture time and ion species are pivotal factors for switching the biological effects of rare earth ions from toxicity to activity,from damage to protection,or from down-regulation to up-regulation.A series of experimental methods including 3-（4,5-dimethyl-2-thiazolyl）-2,5-diphenyl-2H tetrazolium bromide （MTT） test, alkaline phosphatase （ALP） activity measurement, Oil Red O stain and measurement, mineralized function expression and quantitive real time RT-PCR （qRT-PCR） were employed to assess the effect of Nd3＋ and Sm3＋ on the proliferation, differentiation and mineralization function of primary osteoblasts （OBs） in vitro at cell and molecular levels. The experimental results suggest that concentration, culture time and ion species are pivotal factors for switching the biological effects of rare earth ions from toxicity to activity, from damage to protection, or from down-regulation to up-regulation.

关 键 词：成骨细胞 矿化 增殖 体外 分化 PCR检测 作者 钕 

分 类 号：Q813.11[生物学—生物工程] P618.510.5[天文地球—矿床学]