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作 者:罗立津[1] 洪淑珠 吴慧玲[1] 翁华钦 李召虎[1]
机构地区:[1]植物生长调节剂教育部工程研究中心中国农业大学农学与生物技术学院 [2]福建超大现代农业科技研究所
出 处:《科技导报》2010年第15期36-40,共5页Science & Technology Review
摘 要:为验证冠菌素(COR)、脱落酸(ABA)和矿源黄腐酸(MFA)3种调节剂对提高甜椒抗寒性的协同效应,以甜椒为材料,采用砂培法,用三元复配剂(MIXII)和各种单剂进行灌根处理(10mL/株)并于低温(10~15℃)环境下生长60d后,分析低温(5℃,48h)胁迫下,幼苗生长、细胞质膜透性、渗透物质、植株体内活性氧代谢及其抗氧化酶活性的差异。结果表明,复配剂MIXII在低温环境下促进甜椒幼苗生长,同时3种调节剂对抗寒性有一定的协同诱导效应,表现为超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)活性显著提高,从而更加有效地清除体内的活性氧,其效果比使用单剂更为明显;并且MIXII在低温环境下通过ABA的作用提高叶片脯氨酸、可溶性糖等渗透调节物质的含量,来减轻低温胁迫造成的伤害。To study the cooperative inducing effects of coronatine (COR), abscisic acid (ABA), and mineral fulvic acid (MFA) on the chilling resistance of plants, the sandy culture method was used to identify the effects of mepiquat chloride on the lipid peroxidation, electrolyte leakage, reactive oxygen species and antioxidant enzyme activities in sweet pepper (Capsicum annuum) seedlings under cold stress. Sweet pepper seedlings were irrigated with lOmL per plant of COR, ABA, MFA, and their mixture, respectively. The seedlings were planted at temperature of 10-15℃ for 60d, and then cold stress under 5℃ for 48h. The growth of seedlings and the development of lateral roots were promoted by the mixture. The cooperative inducing effects of three plant growth regulators on the chilling resistance is remarkable. Under cold stress, the activities of antioxidant enzymes such as superoxide dismutase (SOD, EC 1,15.1.1), catalase (CAT, EC 1.11.1.6) and peroxidase (POD, EC 1.11.1.7) in leaves were increased. As a result, the efficiency of reactive oxygen species elimination was enhanced. With the higher content of proline and sugar as the effect of abscisic acid in the leaf, the harms of the membrane were relieved by the mixture.
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