高氧液对油酸型急性呼吸窘迫综合征治疗作用机制的实验研究  

The study of hyperoxic liquid therapy for acute respiratory distress syndrome induced by oleic acid

作　　者：王松[1] 许诚[1] 舒丹[1] 段钢[1] 何清[1] 彭建明[2] 洪丰[2] 

机构地区：[1]深圳市东湖医院,深圳518020 [2]暨南大学第一临床学院

出　　处：《中华实验和临床感染病杂志（电子版）》2007年第1期10-13,共4页Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition)

基　　金：深圳市科技局科研基金资助(2003074)

摘　　要：目的探讨静脉输注高氧液(HO)对油酸型急性呼吸窘迫综合征(ARDS)大鼠的作用并探讨其可能机制。方法以林格液为基液制备HO,将30只大鼠随机分为油酸组(A组)、油酸+HO组(B组)和正常对照组(C组),每组各10只。A、B组均经右股静脉注入油酸0.1ml/kg建立ARDS模型,C组注入等量林格液。B组于注入油酸30min后,经由右侧股静脉输注HO(10ml-1.kg-1.h-1);C组输注等量林格液。各组分别于ARDS模型制备前(0min),注入油酸30、60、120min时间点经右颈总动脉采血检测动脉血氧分压(PaO2)、动脉血氧饱和度(SaO2)、动脉血二氧化碳分压(PaCO2)、血液酸碱度(pH)、碱剩余(BE)、碳酸氢根离子(HCO3-);2h后处死动物,取肺组织测定肺组织内丙二醛(MDA)和超氧化物歧化酶(SOD)含量,肺含水量,肺体质量比值及观察其病理变化。结果A、C组比较,A组PaO2显著降低(P<0.01),肺水含量及肺体质量比值显著升高(P<0.01),MDA增加,SOD活力降低(P<0.05);镜下见肺灶性出血,间质、肺泡水肿,大量炎性细胞浸润。A、B组比较,B组经HO治疗后,PaO2迅速提升(P<0.05),肺水肿减轻(P<0.01),MDA降低(P<0.05),SOD活力增加(P<0.05),大部分肺组织镜下见肺损伤明显减轻。结论氧化性肺损伤参与油酸型ARDS发病,HO对油酸型ARDS大鼠有一定的治疗作用,早期干预效果更加。Objective To study the therapeutic effect and mechanism of hyperoxic liquid（HO）therapy for rats with acute respiratory distress syndrome（ARDS）induced by oleic acid.Methods Ringer solution was used as the base solution to prepare HO solution,and 30 rats were randomly divided into 3 groups（10 in each group）：oleic acid group（group A）,oleic acid ＋ HO therapy group（group B）and normal control group（group C）.Group A and group B were injected oleic acid（0.1 ml/kg）via the right femoral vein to set up ARDS models,while group C was injected Ringer＇s solution in the same way.Thirty minutes later,group B was infused HO（10 ml-1 ·kg-1·h-1）via the right femoral vein,while group A and group C were infused with Ringer＇s solution in the same way.Each group was taken blood via the right common carotid artery at 0 min（before injected oleic acid）,30 min,60 min and 120 min（after injected oleic acid）to detect PaO2,SaO2,PaCO2,pH,BE,and HCO3-,respectively.Two hours later,the rats were killed and the lungs were taken out.The concentration of malonaldehyde（MDA）and the activity of superoxide dismutase（SOD）in the lungs were detected.The water content in the lungs and lung/body mass ratio were calculated.The pathological changes of lungs were observed.Results Compared group A with group B,the former had a significant decline of PaO2（P0.01）,a great rise of lung water content and lung/body mass ratio（P0.01）,an increased MDA concentration and a decreased SOD activity（P0.05）;the lung had focus hemorrhage,mesenchymal and alveolus edema,and a great deal of inflammatory cells infiltration when observed by microscopy.Compared group A with group B,the latter had a rapid rise of PaO2（P0.05）,less severe pneumonedema,lower MDA concentration（P0.05）and higher SOD activity（P0.05）after treatment with HO;the injury had a obvious alleviation in the bulk of lung tissue when observed by microscopy.Conclusions The mechanism of ARDS induced by oleic acid may be caused by oxidative inj

关 键 词：高氧液 急性呼吸窘迫综合征 低氧血症 

分 类 号：R563.8[医药卫生—呼吸系统]