维吾尔族D型乙型肝炎病毒株全基因克隆研究  

Analysis of genome sequence of a genotype D strain of HBV from Uighur patient in Xinjiang

作　　者：鲁晓擘[1] 李纲[2] 汤影子[3] 刘霖[3] 张跃新[1] 

机构地区：[1]新疆医科大学第一附属医院感染科,830054 [2]新疆武警总队医院感染科 [3]第三军医大学西南医院感染科

出　　处：《中华实验和临床感染病杂志（电子版）》2007年第1期17-19,共3页Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition)

基　　金：新疆科技厅软科学基金资助项目(编号960505003)

摘　　要：目的进行新疆维吾尔族D型乙型肝炎病毒(HBV)全基因克隆。方法提取慢性HBV患者血清中的病毒DNA,以聚合酶链反应(PCR)对HBV的全基因组进行扩增,克隆入pUCM-T载体,并对测序结果进行比对分析。结果通过HBV全基因克隆化研究,获得我国首例维吾尔族慢性HBV感染者D型HBV病毒株的全基因序列。其基因组全长为3174碱基对(bp),与目前D基因型参照序列比较同源性约为92%～98%,位于1760～1768位出现9个碱基的缺失,与一株来自中国的D/C重组基因型(GenBank登录号:AY862865)序列比较同源性约为91%。氨基酸序列分析其血清型为ayw2型。结论新疆维吾尔族D基因型HBV病毒株有其自身特点。Objective To determine the complete genome sequence of a genotype D strain of HBV from Uighur patient with chronic hepatitis B in Xinjiang.Methods The complete nucleotide sequence of a HBV derived from a Uighur patient with chronic hepatitis B was amplified by PCR,cloned into pUCM-T vector,and analyzed.Results The complete nucleotide sequence of Xinjiang HBV strain is 3174 bp.The complete nucleotide homology is 92%~98% compared with the published sequence of genotype D strains,deletion of 9 bp was found in nt 1760 to nt 1768,91% with the published sequence of genotype D/C recombinat HBV strains from China.This Xinjiang HBV strain belongs to ayw 2 serotype.Conclusions The genome sequence of a genotype D strain of HBV from Uighur patient with chronic hepatitis B was cloned for the first time,which has its own character.

关 键 词：维吾尔族 乙型肝炎病毒 

分 类 号：R512.6[医药卫生—内科学]