艾滋病相关淋巴结病中CD4^+ CD25^+调节性T细胞和增殖细胞核抗原的表达及其关系研究  

The relationship of CD4～+ CD25～+ regulatory T cells and the expression of PCNA in AIDS-related lymphadenopathy

作　　者：兰孟东[1] 马佩卿[1] 赵红心[2] 郎振为[1] 沈冰[1] 王培之[1] 张亮[1] 刀文彬[1] 石晓虹[1] 马志春[1] 张福杰[3] 

机构地区：[1]北京地坛医院病理科,100011 [2]北京地坛医院感染病中心,100011 [3]中国疾病预防控制中心性病艾滋病中心艾滋病治疗与关怀室

出　　处：《中华实验和临床感染病杂志（电子版）》2007年第2期98-101,共4页Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition)

基　　金：首都医学发展科研基金(ZD199914)

摘　　要：目的探讨艾滋病(AIDS)患者淋巴结中CD4+CD25+调节性T细胞及增殖细胞核抗原(PCNA)的表达、分布及其之间的关系。方法对35例AIDS患者的淋巴结进行组织学观察和病理分期,采用免疫组织化学法对淋巴结组织中的PCNA及CD4+CD25+调节性T细胞特异性标记物Foxp3进行检测。结果35例艾滋病患者的淋巴结中,1～4期分别有5、4、14和12例。所有淋巴结组织中均检测出Foxp3及PCNA的表达;在各期淋巴结病变中,Foxp3及PCNA的表达模式相似,在1、2期淋巴结病变中,阳性细胞数量较多,主要分布于滤泡间区和付皮质区;3、4期随着淋巴细胞的数量衰竭,其阳性细胞数量减少更显著。Foxp3与PCNA的表达有显著的正相关。结论艾滋病患者淋巴结中的CD4+CD25+调节性T细胞,随着淋巴结病变的进展和细胞增殖活性的变化,其数量逐渐降低或耗竭。Objective To investigate the expression, distribution and its relationship of CD4＋CD25＋ regulatory T cells and PCNA in lymph nodes from AIDS patients. Methods Thirty-five specimens of lymph nodes from HIV/AIDS patients were examined under microscopy and pathological stages were performed. Specific marker for CD4＋CD25＋ regulatory T cells and PCNA in lymph nodes were detected with anti-Foxp3 monoclonal antibody and anti-PCNA by immunohistochemistry. Results Among 35 cases, there are 5, 4, 14, and 12 cases for histopathological stage from 1 to 4, respectively. Foxp3 and PCNA could be detected in all specimens of lymph nodes. The distributive pattern of Foxp3 is as similar as that of PCNA. Foxp3 and PCNA positive lymphocytes were mainly in intermediate zone of follicle and cortical area in stage 1 and 2. The label index of Foxp3 and PCNA positive lymphocytes was more markedly decreased in stage 3 and 4, following depletion of lymphocytes. The expression of FoxP3 is significantly positive correlation to that of PCNA.Conclusions The CD4＋CD25＋ regulatory T cells in lymph nodes from patients with HIV infection was decreased following the progression of AIDS-related lymphadenopathy and decrease of cell proliferating activation.

关 键 词：调节性T淋巴细胞 增殖细胞核抗原 艾滋病相关淋巴结病 

分 类 号：R512.91[医药卫生—内科学]