HCV F蛋白体外自组装以及在HCV感染者肝组织中的分布研究  

Study on self-assembly of hepatitis C virus F protein in vitro and its distribution in liver of HCV-infected patients

作　　者：邵圣文[1] 于建国[2] 武文斌[3] 赵平[3] 戚中田[3] 

机构地区：[1]湖州大学医学院微生物学教研室,浙江湖州313000 [2]解放军第88医院肝病研究所 [3]第二军医大学微生物学教研室

出　　处：《中华实验和临床感染病杂志（电子版）》2007年第3期138-139,共2页Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition)

基　　金：国家863计划(2006AA02Z401);军队医药卫生"十一五"专项项目(06Z026)

摘　　要：目的探讨HCV F（frameshift）蛋白是否能自组装为病毒样颗粒以及F蛋白是否存在于HCV患者肝组织中。方法将0．5—1g／L大肠埃希菌重组表达的F蛋白与0．006g／L tRNA混合，于100μl组装缓冲液中进行反应，取组装产物进行1：10稀释，2％醋酸铀复染后电镜观察是否形成病毒样颗粒。HCV感染者肝组织中F蛋白的检测采用免疫组织化学法。结果1g／L F蛋白与tRNA混合（167：1）后，可形成直径35nm的病毒样颗粒。在20例HCV感染者的肝穿刺组织标本中，有2例患者的肝细胞胞浆中可检测到F蛋白。结论F蛋白在体外可以形成病毒样颗粒，在部分HCV感染者的肝组织中存在F蛋白，F蛋白在HCV的生活周期中可能有重要功能。Objectives To determine if hepatitis C virus （HCV） F protein self-assemble virion-like particle in vitro and exist in hepatic tissue of HCV-infected patients. Methods After assembly reaction completed by mixing F protein（0. 5 ～ 1 g/L） purified from E. coli with tRNA （0. 006 g/L） in 100μl assembly buffer, product dilution （1/10） was dyed by 2% uranyl acetate and virion-like particle structure was observated under electron microscopy. F protein of hepatic tissue of HCV-infected patients was detected by HRP-SP immunohistochemistry reaction. Results After F protein （ 1 g/L） mixed with tRNA （ 167：1 ）, 35 nm size of virion-like particles formed. Within 20 samples of hepatic tissue section of HCV-infected patients, F protein were found in two samples, both locating in the cytoplasm of hepatic cells. Conclusions F protein can form virion-like particles in vitro, and exist in some hepatic tissue of HCV-infected patients, which indicate F protein may execute some functions in HCV life cycle.

关 键 词：丙型肝炎病毒 F蛋白 病毒样颗粒 肝活检标本 

分 类 号：R512.6[医药卫生—内科学]