益气复脉粉针对内毒素诱导大鼠肠系膜微循环障碍的作用研究  

Effects of Yiqi Fumai injection （益气复脉粉针） on lipopolysaccharide-induced mesentery microcirculatory disturbance in rats

作　　者：原庆[1] 方秋红[1] 马迎民[1] 韩晶岩[2] 张淑文[3] 

机构地区：[1]北京世纪坛医院呼吸内科,北京100038 [2]北京大学医学部微循环研究中心,北京100083 [3]首都医科大学附属北京友谊医院感染内科,北京100054

出　　处：《中国中西医结合急救杂志》2010年第4期221-225,共5页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

摘　　要：目的探讨静脉给予益气复脉粉针对内毒素血症大鼠肠系膜微循环障碍的改善作用。方法将30只Wistar大鼠随机分为对照组、模型组、治疗组，每组10只。静脉注入脂多糖（LPS）10mg·kg-1·h-1复制内毒素血症微循环障碍模型，20min后治疗组连续静脉注射益气复脉粉针（80mg·kg-1·h-1），对照组和模型组给予等量生理盐水。每20min动态观察大鼠肠系膜细静脉壁黏附白细胞数、细静脉壁过氧化物以及白蛋白渗出的变化，直至100min结束观察。检测肠系膜间质内肥大细胞脱颗粒率，用流式细胞仪测定外周血粒细胞黏附分子CD11b和CD18的表达，用免疫组化法检测肺组织细胞间黏附分子-1（ICAM-1）及核转录因子-KB（NF—kB）p65的表达。结果20min起模型组大鼠肠系膜细静脉壁黏附白细胞数、管壁过氧化物依存的二氢罗达明（DHR）荧光强度和白蛋白渗出均明显增加（均P〈0．05）；100min后肠系膜间质内肥大细胞脱颗粒率亦显著增加，外周血粒细胞黏附分子CD11b和CD18的表达明显增加，肺组织ICAM-1、NF—kBp65积分吸光度（A）值明显升高（均P〈0．05）。与模型组相比，治疗组40min后细静脉壁黏附自细胞数明显减少，细静脉壁过氧化物依存的DHR荧光强度增加明显受抑制，白蛋白渗出明显减少（均P〈0．05）；100min后肠系膜间质内肥大细胞脱颗粒率明显减少，外周血粒细胞黏附分子CD11b和CD18表达明显下降，肺组织ICAM-1、NF—kBp65积分A值明显降低（均P〈0．05）。结论益气复脉粉针对LPS诱导的肠系膜微循环障碍有改善作用，机制可能与其能解离白细胞与血管内皮黏附的作用有关，而该作用又可能与减少粒细胞黏附分子CD11b和CD18、血管内皮ICAM-1及NF—kBp65表达有关。Objective To explore the effect of intravenous infusion of Yiqi Fumai lyophilized preparation （益气复脉粉针） on lipopolysaccharide （LPS） induced mesentery microcirculatory disturbance in rats. Methods Thirty Wistar rats were randomly divided into control group, model group and treatment group （each n= 10）. The rat model of septic mesenteric microcirculatory impairment was established by infusing with LPS 10 mg · kg-1· h-1 via the left femoral vein for 100 minutes. The rats in treatment group was treated with Yiqi Fumai intravenous injection （80 mg · kg-1 · h-1） 20 minutes after the model establishment. Equal quantity of normal saline was administered in control and model groups. By virtue of a microcirculatory observation system, the changes of number of adherent leukocytes on the mesentery venule, peroxide in venular wall and albumin exudates were determined every 20 minutes until 100 minutes of LPS infusion. The rate of mast cell degranulation in the mesentery interstitial tissue was counted. Afterwards, the peripheral blood was collected. Flow cytometry and fluorescence methods were applied to determine the expressions of granulocyte adherent molecules CD11b and CD18 in the peripheral blood. Immunohistochemical method was used to evaluate the expressions of intercellular adhesion molecule-1 （ICAM-1） and nuclear factor-kB （NF-kB） p65 in the lung tissue. Results In the model group, after 20 minutes of LPS infusion, the number of leukocytes adherent to mesentery venutar wall, the intensity of hydrogen peroxide （H2O2） dependent dihydrorhodamine 123 （DHR） fluorescence in the venular walls and albumin leakage from venules were increased obviously （all P〈0. 05）. After 100 minutes, The rate of mast cell degranulation in the mesentery interstitial tissue was also increased markedly, the granulocyte expressions of adherent molecules CD11b and CD18 were obviously increased in the peripheral blood, and the integral absorbance （A） values of ICAM-1 and NF-kBp65 in lung tis

关 键 词：益气复脉粉针 脂多糖 微循环障碍 白细胞黏附 肥大细胞脱颗粒 黏附分子 

分 类 号：R285.5[医药卫生—中药学] R572.3[医药卫生—中医学]