TUNEL法检测类猪圆环病毒2型因子P1诱导猪免疫器官细胞凋亡的研究  

Apoptosis in Immune Organs of Pigs Induced by Porcine Circovirus Type 2-like Agent P1

作　　者：温立斌[1] 何孔旺[1] 杨汉春[2] 王玉然 董美响 

机构地区：[1]江苏省农业科学院兽医研究所,江苏南京210014 [2]中国农业大学动物医学院,北京100094 [3]河北省动物卫生监督所,河北石家庄050081 [4]河北省饲料工业协会,河北石家庄050011

出　　处：《华北农学报》2008年第B10期88-91,共4页Acta Agriculturae Boreali-Sinica

基　　金：国家“973”计划前期研究专项资助课题(2007CB116308);江苏省博士后基金(5910602)

摘　　要：研究类猪圆环病毒2型因子P1体内诱导的细胞凋亡作用。将P1分子克隆接种普通仔猪,用原位末端标记技术(TUNEL)检测猪的扁桃体、脾脏、腹股沟淋巴结等免疫器官细胞的凋亡情况。猪感染P1后21和35 d的扁桃体、脾脏和腹股沟淋巴结等免疫器官细胞出现了凋亡。P1可能引起猪的免疫抑制。The research is to study the apoptosis of pigs induced by porcine circovirus type 2-like agent P1.The apoptosis was detected by TUNEL assay in immune organ samples（tonsil,spleen,superficial inguinal lymph node） of pigs infected by P1 molecular clone.The results showed that the apoptosis-specific signal was detected in immune organ samples（tonsil,spleen,superficial inguinal lymph node） at 21 and 35 d after P1 infection.The result suggests that P1 appears to be related to immunosuppression resulted in apoptosis of lymphocyte.

关 键 词：猪圆环病毒2型 P1 凋亡 TUNEL 

分 类 号：S852.65[农业科学—基础兽医学]