Ca2＋·CaM信使系统参与小麦抗叶锈病反应的初步研究  

Preliminary Research on Ca^(2+)·CaM Involved in the Resistance Response of Wheat to Puccinia triticina

作　　者：霍建飞[1,2] 宋水山[1,3] 李星[1] 杨文香[1] 付胜杰[1] 刘大群[1] 

机构地区：[1]河北农业大学植物保护学院植物病理系河北省农作物病虫害生物防治工程技术研究中心,河北保定071001 [2]天津市植物保护研究所,天津300112 [3]河北省生物研究所,河北石家庄050051

出　　处：《华北农学报》2008年第B10期234-239,共6页Acta Agriculturae Boreali-Sinica

基　　金：国家“973”前期项目(2005CCA01600)

摘　　要：研究Ca2+.CaM是否参与小麦抗叶锈病反应的过程,为更深入了解小麦抗叶锈病的反应机制奠定基础。采用离体培养的方法测定小麦种子经CaM拮抗剂TFP(三氟拉嗪)、CPZ(氯丙嗪)和W-7(N-(6-aminohexyl)-5-chloro-1-naphthalene sulfonamide)以及CaCl2预处理后小麦叶片接种叶锈菌后在不同时间点的酶活性。另外用实时定量PCR的方法检测小麦接种亲和小种和非亲和小种后CaM不同亚型基因的表达情况。试验结果表明:小麦叶片接种非亲和性叶锈菌后,TFP、CPZ和W-7浸种处理抑制了过氧化物酶(POD)、丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)和过氧化氢酶(CAT)活性的上升;小麦叶片接种亲和性叶锈菌后,CaCl2预处理加剧了过氧化物酶(POD)、丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)和过氧化氢酶(CAT)活性的上升。这说明Ca2+.CaM信使系统可能在小麦抗叶锈病过程中起着重要作用,并且不同的CaM亚型可能调控不同的抗病途径。Investigate if CaM is involved in the defense responses of wheat to Puccinia triticina,and provide more evidences to the next research.After being blocked Ca2＋·CaM messenger transduction by pretreating with CaM antagonists TFP（trifluoperazine）,CPZ（chlorpromazine） and W-7 [（N-（6-aminohexyl）-5-chloro-1naphathalene sulfonamide）]and activated with pretreatment of CaCl2,the activities of peroxidase（POD）,phenylalanine ammonialyade（PAL）,polyphenoloxidase（PPO） and catalase（CAT） were measured in wheat leaves inoculated with Puccinia triticica.Addition,the expression of the CaM gene was tested by Real-Time Fluorescent Quantitative PCR after inoculating virulent race and avirulent race respectively.The results showed that the pretreatment with CaM repel reagents TFP,CPZ and W-7 inhibited the increase in the activities of POD,PAL,PPO and CAT after the inoculation with 05-5-127③ in detached wheat leaves.The pretreatment with CaCl2 enhanced the increase in the activities of POD,PAL,PPO and CAT after the inoculation with 05-22-64① in detached wheat leaves.And there is differential expression of CaM isforms genes under different interactions in wheat after inoculation.The results indicated that Ca2＋·CaM messenger might play an important role in the interaction of wheat and pathogen,and different CaM isforms might play different role.

关 键 词：小麦 叶锈菌 Ca2+·CaM 

分 类 号：S435.12[农业科学—农业昆虫与害虫防治]