烟草WS38生长素结合蛋白基因cDNA的克隆及序列分析  

Cloning and sequence analysis of auxin-binding proteins gene cDNA of Nicotiana tabacum WS38

作　　者：刘晓柱[1] 黄妤[1] 彭珍子[1] 周晶辉[1] 洪亚辉[1] 张学文[1] 

机构地区：[1]湖南农业大学生物科学技术学院,湖南长沙410128

出　　处：《湖南农业大学学报（自然科学版）》2010年第4期388-391,共4页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：湖南省自然科学基金项目(08JJ5020)

摘　　要：以烟草WS38为材料,根据GenBank中登录的烟草生长素结合蛋白(ABP1)氨基酸序列设计引物,通过RT-PCR克隆了烟草WS38ABP1基因cDNA分子编码区,序列全长为564bp,可编码1条188个氨基酸的多肽.利用ClustalX软件对该序列翻译获得的多肽序列与报道的烟草生长素结合蛋白氨基酸序列比较,两者同源性为98.4%,存在2个氨基酸的差异,但差异氨基酸对ABP1结构和功能影响微弱.认为克隆的cDNA序列即为烟草WS38ABP1cDNA,不同品种烟草ABP1基因存在的核苷酸单位点多态性(SNP)造成了两者氨基酸序列的差异.Nicotiana tabacum WS38 ABP1 cDNA coding sequence was cloned by RT-PCR with the primers that were designed according to reported Nicotiana tabacum ABP1 amino acid sequence in GenBank. The sequence analysis showed that the length of the cDNA was 564 bp. Information analysis indicated that the open reading frame can encode 188 amino acids polypeptide with the 98.4% homology with the reported ABP1. It also demonstrated that the ABP1 in Nicotiana tabacum varied in different cultivars and there was some single nucleic acid polymorphasim with WS38 and NK326.

关 键 词：烟草 生长素结合蛋白 CDNA克隆 序列分析 

分 类 号：Q785[生物学—分子生物学]