巯基修饰单链DNA在纳米金薄膜电极上自组装、杂交和取向的电化学交流阻抗谱  

Electrochemical Impedance Spectroscopy Study of SH-ssDNA Self-Assembling,Hybridization and Orientation on Thin-film Nana-gold Electrode

作　　者：魏旭[1] 郝青丽[1] 陆路德[1] 汪信[1] 杨绪杰[1] 

机构地区：[1]南京理工大学材料化学实验室,南京210094

出　　处：《应用化学》2010年第8期970-977,共8页Chinese Journal of Applied Chemistry

基　　金：江苏省科技支撑计划(BE2009159;BK2006201);南京理工大学卓越计划紫金之星(2008)资助项目

摘　　要：利用电化学交流阻抗技术对SH-ssDNA在纳米金薄膜电极表面的自组装、杂交和取向进行了系统表征。探讨了SH-ssDNA的组装时间、浓度和链长对其自组装的影响,自组装15h时电荷传递电阻Rct最大,表面覆盖率最高;研究了SH-ssDNA的浓度、链长以及与互补DNA的杂交方式对杂交反应的影响。结果发现,随着单链浓度的增加,杂交后Rct的变化值逐渐降低,当SH-ssDNA为5μmol/L时Rct值比杂交前增加了16%。通过对阻抗谱数据模拟和分析,表明SH-ssDNA以垂直竖立取向在金电极表面形成均匀致密单分子层,杂交效率与SH-ssDNA的覆盖率密切相关。The self-assembling,hybridization and orientation of SH-ssDNA on the surface of thin-film nano- gold electrodes were characterized systematically by means of the electrochemical impedance technique. The effects of assembling time,concentration and the length of SH-ssDNA on its self-assembling were investigated. The Rct and surface coverage reached the highest values when assembling time was 15 h. The influences of the concentration and length of SH-ssDNA and its hybridization method with complementary DNA on the hybridization reaction were also studied. The result shows the change of Rct decreases with the increase of the SH-ssDNA concentration. The Rct was increased by 16% when the concentration of SH-ssDNA was 5 μmol /L. The data simulation and analysis of impedance spectroscopy indicated that a unique self-assembled monolayer of SH-ssDNA was formed with an upright orientation on the nano-Au electrode surface. The result shows the hybridization efficiency is closely associated with the coverage of SH-ssDNA.

关 键 词：交流阻抗谱 SH-ssDNA 自组装 杂交 取向 

分 类 号：O657.1[理学—分析化学]