人hsp90β基因的热诱导表达与染色质活化的关系  

Distribution of Human hsp90β Gene in Active Chromatin Upon Heat Shock

作　　者：施一江[1] 沈珝琲 

机构地区：[1]中国医学科学院中国协和医科大学基础医学研究所医学分子生物学国家重点实验室

出　　处：《中国医学科学院学报》1999年第2期126-129,共4页Acta Academiae Medicinae Sinicae

基　　金：国家自然科学基金

摘　　要：目的研究人ｈｓｐ９０β基因的热诱导表达与染色质活化的关系。方法采用有机汞亲和层析柱分离热诱导前后Ｊｕｒｋａｔ细胞内的活性和非活性染色质，然后采用狭缝杂交技术比较热诱导前后人ｈｓｐ９０β基因在活性和非活性染色质中的分布，并用Ｎｏｒｔｈｅｒｎ印迹杂交的方法研究热休克诱导下的ｈｓｐ９０βｍＲＮＡ表达。结果发现热休克诱导使细胞内人ｈｓｐ９０β基因在活性染色质中的含量明显增加，并与内源性的ｈｓｐ９０βｍＲＮＡ表达水平一致。结论首次证实人ｈｓｐ９０β基因的热诱导表达在染色质水平受“基因开关”的调控，为研究人类细胞热应激机制提供了新的模型。Objective To study the distribution of human hsp90β gene in active chromatin of Jurkat cells subjected to heat shock.Methods Active and inactive chromatin were isolated by using organomercurial affinity chromatography from Jurkat cells with or without heat shock. Slot blot hybridization was then carried out with human hsp90β cDNA probe.And the efficiency of mRNA expression was studied by Northern blot hybridization.Results The content of the hsp90β gene distributed in the active chromatin was increased upon heat shock which was comparable with the highly efficient mRNA expression induced by the same treatment.Conclusions The results suggested chromatin activity was a prerequisite for heat shock induction of hsp90β gene.

关 键 词：HSP90Β基因 亲和层析 活性染色质 染色质 CDNA 

分 类 号：Q523[生物学—生物化学]