甲醛炎性痛诱导脊髓后角HO-1蛋白表达改变及其对脊髓神经元凋亡的影响  

Effect of HO-1 expression on neuronal apoptosis in spinal cord of rats with formaldehyde inflammatory pain

作　　者：宋延辉[1] 胡玉燕[2] 李清君[2] 李文斌[2] 陈瑞红[2] 赵燕[2] 

机构地区：[1]广州金域医学检验中心病理室,广东广州510330 [2]河北医科大学病理生理学教研室,河北石家庄050017

出　　处：《中国病理生理杂志》2010年第8期1589-1592,共4页Chinese Journal of Pathophysiology

基　　金：河北省科技攻关课题资助项目(No.06276102D-40)

摘　　要：目的:观察甲醛炎性痛大鼠脊髓后角HO-1蛋白表达改变以及HO-1对炎性痛大鼠脊髓神经元凋亡的影响。方法:采用Western blotting方法检测左、右两侧脊髓后角HO-1蛋白的表达,采用流式细胞技术检测脊髓神经元凋亡率。结果:与对照组相比,足底注射甲醛后HO-1蛋白表达明显上调,其中以注射甲醛后24 h升高最为明显,72 h仍高于对照组水平。左、右两侧脊髓后角HO-1蛋白表达无明显差异。与对照组比较,甲醛组大鼠脊髓神经元凋亡率明显升高;鞘内预先注射HO-1抑制剂ZnppIX可使甲醛诱导的自发痛反应程度明显降低,并可使甲醛炎性痛大鼠脊髓神经元凋亡率进一步升高。结论:甲醛炎性痛可诱导大鼠脊髓后角HO-1蛋白表达升高,并可诱导脊髓神经元发生凋亡,甲醛炎性痛诱导的HO-1表达增强在脊髓伤害性信息传递过程中起促进作用;在炎性痛诱导的脊髓神经元凋亡过程中起抑制作用。AIM： To observe the change of heme oxygenase（HO-1）expression in the spinal cord during formaldehyde-induced pathological pain and investigate the effect of HO-1 on neuronal apoptosis in the spinal cord induced by formaldehyde inflammatory pain.METHODS： The protein expression of HO-1 in the left and the right spinal dorsal horn was detected by Western blotting.The neuronal apoptosis rate of the spinal cord was determined by flow cytometry.RESULTS： Compared to control group,the protein expression of HO-1 was significantly up-regulated at different time points after the injection of formaldehyde,which was most obviously 24 h after the injection of formaldehyde and was still higher than that in control group at 72 h.Compared to control group,the neuronal apoptosis rate of spinal cord increased in the rats with formaldehyde inflammatory pain.No significant difference of the neuronal apoptosis rate was observed between formaldehyde group and solvent control group[intrathecal injection of dimethyl sulfoxide（DMSO）,the solvent of zinc protoporphyrin（ZnppIX）].Intrathecal injection of 100 μg ZnppIX,an inhibitor of HO-1,attenuated the degree of spontaneous pain response,but induced an increase in the rate of neuronal apoptosis in spinal cord in the rats with formaldehyde inflammatory pain.CONCLUSION： Formaldehyde inflammatory pain induces the increases in HO-1 expression and neuronal apoptosis in the rat spinal cord.HO-1 promotes the response of spontaneous pain and inhibits the process of neuronal apoptosis in spinal cord induced by formaldehyde inflammatory pain.

关 键 词：甲醛 疼痛 神经元凋亡 脊髓 血红素氧合酶 

分 类 号：R338.3[医药卫生—人体生理学]