人早孕绒毛滋养层细胞的分离纯化及马鞭草抗早孕机理的初步研究  

TROPHOBLAST CELLS ISOLATED FROM HUMAN VILLI OF EARLY PREGNANCY AND STUDY ON MECHANISM OF ABORTIFACIENT EFFECT BY VERBENA OFFICINALIS

作　　者：徐昌芬[1] 卢小东[1] 周亚东[1] 焦中秀[1] 祝辉[1] 朱启锭[1] 

机构地区：[1]南京医科大学组织胚胎学教研室,南京210029

出　　处：《解剖学杂志》1999年第2期137-140,共4页Chinese Journal of Anatomy

基　　金：江苏省科委资助项目!(BJ95116)

摘　　要：目的 :探讨马鞭草抗早孕的细胞学作用机理。方法 :正常妊娠 6～ 8周早期胎盘 Percoll梯度离心分离出滋养层细胞进行体外培养 ,观察马鞭草对培养的滋养层细胞形态及绒毛膜促性腺激素分泌功能的影响。结果 :2 5、50、mg/ ml马鞭草乙醇提取液对滋养层细胞生长及绒毛膜促性腺激素分泌有明显的抑制作用。结论 :一定浓度的马鞭草可直接杀伤滋养层细胞 。Objective: To study the mechanism of abortifacient effect of Verbena Offcinalis. Methods: Trophoblast cells were isolated from human villi of early pregnancy by percoll gradient centrifugation and cultured in vitro. We observed the morphological changes of human trophoblast cells as well as the HCG secretion. Results: Verbena Offcinalis at 25 mg/ml inhibited not only cells growth but also HCG secrection significantly. Conclusion: Verbena Offcinalis of certain concentrations directly damaged trophoblast cells and inhibited HCG secretion. The study provided some theoretical evidence for the clinical abortifacient applications of Verbena Offcinalis.

关 键 词：滋养层细胞 马鞭草 HCG 早孕 终止妊娠 中药 

分 类 号：R169.42[医药卫生—公共卫生与预防医学] R286.92