怀山药的离体繁殖  

n vitro Propagation of Common Yam (Dioscorea opposita)

作　　者：李明军[1] 李金亭[1] 朱命炜[1] 张嘉宝[1] 

机构地区：[1]河南师范大学生物系,新乡453002

出　　处：《中草药》1999年第4期296-298,共3页Chinese Traditional and Herbal Drugs

基　　金：河南省自然科学基金;省科技攻关项目

摘　　要：怀山药带节茎段在 ＭＳ＋６－ＢＡ　１～２ｍｇ／Ｌ＋ＮＡＡ ０．１～１ｍｇ／Ｌ的培养基上培养，均能直接形成多芽体，均芽数为 ３～５。将多芬体转入ＭＳ或 ＭＳ＋ＫＴ ２ｍｇ／Ｌ＋ＮＡＡ ０．０２ ｍｇ／Ｌ＋ＰＰ_３３３ ０．１ｍｇ／Ｌ的培养基上均能诱导生根，形成再生植株。但后者形成的苗生长健壮，叶色浓绿，有较强的生活力。Dioscorea opposita Thunb. was micropropagated from nodal culture. This species produced 3 ~5 buds per node when cultured on modified MS Medium supplemented with 6-BA 1-2mg/L and NAA 0.1 --1 mg/L. Root could be induced when these multibuds were transplanted onto MS basic Medium or MS basic Medium supplemented with KT 2 mg/L, PP333 0.1 mg/L and NAA 0.02 mg/L. The regenerated plantlets treated with PP333, grew robustly and developed deep green leaves with vigour.

关 键 词：怀山药 节培养 多芽体 再生植株 离体繁殖 

分 类 号：S567.239[农业科学—中草药栽培] S632.103.5[农业科学—作物学]