乙酰胆碱对大鼠B淋巴细胞功能的作用  

EFFECT OF ACETYLCHOLINE ON B-LYMPHOCYTE FUNCTION OF RATS

作　　者：邱一华[1] 彭聿平[1] 程纯[1] 戴丽[1] 黄慧伟[1] 

机构地区：[1]南通医学院生理学教研室

出　　处：《南通医学院学报》1999年第2期135-137,共3页ACTA Academiae Medicinae Nantong

摘　　要：目的：实验观察不同浓度乙酰胆碱（ＡＣｈ，１０－１０～１０－５ｍｏｌ／Ｌ）对大鼠Ｂ细胞功能的作用，并初步探讨其作用机制。方法：在离体条件下，利用细胞培养方法，以绵羊红细胞（ＳＲＢＣ）刺激大鼠肠系膜淋巴结Ｂ细胞转化成抗体形成细胞（ＡＦＣ），然后检测其抗体生成量，并以此作为衡量Ｂ细胞功能的指标。结果：（１）１０－１０～１０－７ｍｏｌ／ＬＡＣｈ能显著抑制Ｂ细胞功能，其中１０－８和１０－７ｍｏｌ／ＬＡＣｈ的作用较强，而１０－６和１０－５ｍｏｌ／ＬＡＣｈ无明显的抑制作用。（２）Ｍ型胆碱能受体拮抗剂阿托品（１０－７和１０－６ｍｏｌ／Ｌ）可完全阻断ＡＣｈ抑制Ｂ细胞功能的作用。结论：ＡＣｈ可非浓度依赖性地抑制大鼠的Ｂ细胞功能，此作用可能由Ｂ细胞上的Ｍ型胆碱能受体介导。Objective: In the present study, the effect of acetylcholine (ACh) on B-lymphocyte function and the primary mechanism of the action were investigated by means of the assay for antibody synthesis of rats in vitro. Methods:TBZ] Various concentrations of ACh (10-10 to 10-5 mol/L) and sheep red blood cells (SRBC) were added to the lymphocyte suspension of the mesenteric lymphaden of rats and incubated for five days. Then the optical density (OD) value showed that the quantity of antibody synthesis of B-lymphocyte was determined.X] Results: ACh at 10-10 to 10-7 mol/L range significantly suppressed the antibody synthesis in vitro, having a stronger action at 10-8 and 10-7 mol/L. ACh at 10-6 and 10-5 mol/L had not remarked effect on the antibody synthesis in vitro. Atropine, a muscarinic receptor antagonist, abolished completely the inhibitory effect of ACh on the antibody synthesis in vitro. Conclusion: These results suggest that ACh can cause concentration-independent suppression of the antibody synthesis of rats in vitro, and the action of ACh may be mediated by the muscarinic receptor.

关 键 词：乙酰胆碱 B淋巴细胞 神经递质 抗体 神经免疫调节 

分 类 号：R338[医药卫生—人体生理学] R392.12[医药卫生—基础医学]