骨髓间质干细胞经静脉移植对大鼠脊髓损伤后TNF-α、IL-1β mRNA表达的影响  

Influence of Intravenous Administration of Bone Marrow Mesenchymal Stem Cells on Expression of TNF-α,IL-1β mRNA after Spinal Cord Injury in Rats

作　　者：董锋[1] 林建华[1] 吴朝阳[1] 

机构地区：[1]福建医科大学附属第一医院骨科,福建省福州市350005

出　　处：《中国骨与关节损伤杂志》2010年第8期697-699,共3页Chinese Journal of Bone and Joint Injury

基　　金：教育部科学技术研究重点项目(01058);福建省科技厅重点项目(2002Y014);福州市科技局项目(2002-14)

摘　　要：目的研究大鼠骨髓间质干细胞(MSCs)经静脉移植对脊髓损伤(SCI)后肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)表达的影响。方法运用改良Allen法制备大鼠脊髓外伤性截瘫模型,假手术组6只,损伤组84只随机分为对照组和rMSCs移植组。对照组和rMSCs移植组、假手术组于损伤后10min经尾静脉分别注射PBS1ml和rMSCs单细胞PBS悬液1ml。移植后3h、6h、12h、24h、3d、7d、14d,应用RT-PCR方法检测损伤大鼠脊髓TNF-α、IL-1βmRNA表达变化情况。结果对照组和rMSCs移植组损伤脊髓TNF-α、IL-1βmRNA表达较假手术组有明显增加(P<0.05);rMSCs移植组与对照组比较,TNF-α、IL-1βmRNA表达受到明显抑制(P<0.05)。结论 rMSCs静脉移植后能改变脊髓损伤区的微环境,使损伤脊髓局部的TNF-α、IL-1β表达程度降低。这可能是减少脊髓继发性损伤,促进损伤大鼠运动功能恢复的机制之一。Objective To explore the influence of intravenous administration of bone marrow mesenchymal stem cells on expression of tumor necrosis factor-α （TNF-α）,interleukin-1β（IL-1β） mRNA after spinal cord injury in rats .Methods Rats were subjected to a weight driven implant injury. There were 6 in the non-injuried group and 84 rats in the injuried group. Then the injuried group was randomly divided into transplantati-on group and control group.There were 7 time points in each group,n=6 in each point）.rMSCs or PBS were infused into tail vein at 10 minutes after injury.Results Expression of TNF-ot, IL-1β mRNA in the rMSCs group and in the control group was higher than that in non-injured spinal cord sections （P〈0.05）.Compared with the control group,expression of TNF-ct,IL-1βmRNA in the rMSCs group was lower significantly （P〈0.05）; Conclusion rMSCs can downregulate TNF-α,IL-1β of the injured area of spinal cord.Maybe it is one of the mechanism of decreasing the secondary spinal cord injury , improving motor function in SCI rats. Key words Spinal cord injury;Bone marrow mesenchymal stem cells; Interleukin -1β;Tumor necrosis factor-α;Intravenous transplantation

关 键 词：脊髓损伤 骨髓间质干细胞 TNF-Α IL-1Β 大鼠 静脉移植 

分 类 号：R714.502[医药卫生—妇产科学]