青花菜查尔酮合成酶基因BoCHS的克隆、表达及序列分析  

Cloning,Expression and Sequence Analysis of a Chalcone Synthase Gene BoCHS from Brassica oleracea var. italica

作　　者：彭礼琼[1] 蒋明[1] 郭志平[2] 章鲤静[1] 张徐俞[1] 

机构地区：[1]台州学院生命科学学院,浙江临海317000 [2]丽水学院化学与生命科学学院,浙江丽水323000

出　　处：《湖北农业科学》2010年第8期1796-1800,共5页Hubei Agricultural Sciences

基　　金：浙江省自然科学基金项目(Y3080081);台州市科技计划项目(08XH02)

摘　　要：根据NCBI数据库中发布的查尔酮合成酶基因序列设计PCR简并引物,以青花菜(Brassica oler-acea var.italica)子叶cDNA为材料,克隆到了青花菜查尔酮合成酶基因,基因命名为BoCHS,序列已提交到NCBI数据库,登录号为GU266209。该基因的编码区全长为1182bp,编码393个氨基酸。序列比对结果表明,BoCHS基因编码的氨基酸序列与其他十字花科植物之间的同源性很高,仅存在个别氨基酸残基的差别。RT-PCR检测表明,经霜霉病菌(Hyaloperonospora parasitica)侵染后,子叶中BoCHS的表达量增加,其中以72h和96h的表达量最大。Degenerate primer pairs were designed according to chalcone synthase gene sequences published by NCBI.The gene, designated BoCHS, was successfully isolated from cotyledon cDNA of Brassica oleracea var.italica.Gene sequence has been submitted to NCBI with an accession number of GU266209.Complete coding sequence of BoCHS was 1 182 bp in length encoding 393 amino acids.Sequence comparison results showed that BoCHS shared high homology with those of crucifer plants and there were only few amino acid residues differences.RT-PCR results indicated an increase gene expression in Hyaloperonospora parasitica infected cotyledons and revealed a significant increase at 72 h and 96 h of treatment.

关 键 词：青花菜 查尔酮合成酶基因 序列分析 基因表达 

分 类 号：S635.3[农业科学—蔬菜学] Q559.1[农业科学—园艺学]