通过HIT_(8a)和HIT_(8b)两个单克隆抗体鉴定发现CD_8抗原有不同的表位  

DIFFERENT EPITOPES IN CD8 ANTIGEN DEFINED WITH TWO MONOCLONAL ANTIBODIES HIT8a AND HIT8b

作　　者：沈德诚[1] 陈璋[1] 白金芬[1] 佘鸣[1] 陈辉树[1] 孙同哲[1] 汤美华[1] 段世利[1] 卞钟元 张金香[1] 杨希峰[1] 

机构地区：[1]中国医学科学院血液学研究所,天津300020

出　　处：《上海免疫学杂志》1990年第3期147-150,共4页Shanghai Journal of Immunology

摘　　要：用间接免疫荧光法、免疫组化技术鉴定了HIT_(8a)和HIT_(8b)两个单克隆抗体(单抗)对各种正常组织、细胞系和各种白血病患者细胞的反应性,证明其反应模式与CD8单抗相似。HIT_(8a)、HIT_(8b)识别的胸腺细胞上抗原分子量分别为 59和60KD(未还原),也与CD8近似。然而,竞争抑制试验显示,9个国际命名的 CD8单抗中只有5个可以完全抑制 HIT_(8a)、HIT_(8b)-FITC与靶细胞结合,4个无作用。证实HIT_(8a)和 HIT_(8b)是 CD8样抗体,识别同一抗原位点。但也揭示 CD8抗原有不同表位。本文对此进行了讨论。The reactive pattern of monoclonal antibodies (McAbs) HIT8a and HIT8b in normal cells, leukemic cell lines and various leukemia patient cells is considered to be similar to CD8 McAb by indirect immunofluorescence and immunohistochemistry techniques. The antigens recognized by HIT8a and HIT8b McAbs have molecular weight of 59KD and 60KD (non-reduced) respectively also close to that of CD8. However, competitive binding assay showed that the reaction of McAbs HIT8a and HIT8b labeled with FITC could be inhibited completely only by 5 out of the 9 CD8 McAbs named by international conference and the remaining 4 were without blocking effect. The results showed that HIT8a and HIT8b were CD8-like McAbs, recognizing the same epitope, and also suggested that there were different epitopes in CD8 antigen.

关 键 词：单克隆抗体 鉴定 CD8 抗原 

分 类 号：R392－33[医药卫生—免疫学]