2种冻融方法对人类早期胚胎质量的影响  

Effect of the vitrification and slow freezing cryopreservation on the quality of human embryos

作　　者：谢晓东[1] 王洪华[1] 熊芳[1] 

机构地区：[1]南京医科大学附属无锡妇幼保健院生殖医学科,江苏省214002

出　　处：《中华实用诊断与治疗杂志》2010年第8期772-774,共3页Journal of Chinese Practical Diagnosis and Therapy

摘　　要：目的:探讨2种冷冻方法对人类早期胚胎冷冻复苏后胚胎质量的影响。方法:回顾性分析本院胚胎实验室胚胎冷冻复苏资料,依据胚胎冷冻方法不同分为玻璃化冷冻组(130枚)和程序化冷冻组(120枚),分析比较2组胚胎复苏率、复苏胚胎完整率、优质胚胎率、临床妊娠率等数据。结果:玻璃化冷冻复苏组与程序化冷冻组胚胎复苏率分别为100.0%与83.3%,复苏胚胎完整率为96.2%与63.5%,优质胚胎率为76.7%与50.0%,临床妊娠率为54.6%与33.3%,2组比较差异有统计学意义(P<0.05)。结论:玻璃化冷冻技术可简化胚胎冷冻程序,冷冻复苏效果显著,可提高临床妊娠率。Objective To investigate the effect of the vitrification and the slow freezing cryopreservation on the quality of human cleavage-stage embryos.Methods A retrospective study was performed by analyzing the human embryo data from our hospital.There were 130 embryos in the vitrification group and 120 embryos in the conventional freezing group.The survival rate,blastomere integrity rate,high-grade rate and clinical pregnancy rate were compared between the two groups.Results The survival rates were 100.0% and 83.3% respectively in the vitrification group and the control group.The blastomere integrity rates were 96.2% and 63.5% respectively,the high-grade rates were 76.7% and 50.0%,and the clinical pregnancy rates were 54.6% and 33.3% respectively,which showed significant differences between the two groups（P0.05）.Conclusion The vitrification technique can simplify the procedure,and significantly improve the high cryopreservation and survival effect as well as the clinical pregnancy rate.

关 键 词：玻璃化冷冻 胚胎 复苏率 妊娠率 

分 类 号：R321-33[医药卫生—人体解剖和组织胚胎学]