用基因枪转化花粉获得转基因谷子和玉米  

Production of Transgenic Millet and Maize Plants by Particle Bombardment

作　　者：董云洲[1,2,3] 段胜军[1,2,3] 赵连元 杨秀海[1,2,3] 贾士荣 

机构地区：[1]中国农业科学院生物技术研究中心 [2]河北省农林科学院谷子研究所 [3]中国农业科学院作物育种栽培研究所

出　　处：《中国农业科学》1999年第2期9-13,共5页Scientia Agricultura Sinica

摘　　要：以国产基因枪ＪＱ－７００分别将ＧＵＳ（β－葡糖醛酸苷酶）基因射入谷子和玉米的花粉，经人工授粉获得种子。在加卡那霉素（Ｋａｎ）的培养液中筛选获得Ｋａｎ抗性植株。对抗性植株进行ＧＵＳ组织化学和Ｓｏｕｔｈｅｒｎ杂交检测，结果表明有外源基因的整合与表达。转基因谷子和玉米的频率分别为０．１８％和０．０５％～０．２１％。Bombardment to both millet and maize pollen grains with plasmid pBI121 containing kanamycinresistant gene and GUS gene were performed. After bombardment , pollens of Jigu No.11 millet were pollinated onto the stigma of a male sterile line 91045. Seeds were harvested and germinated in a selective nutrient solution contaning kanamycin 50 mg/L. GUS assay and Southern hybridization of kanamycinresistant seedlings indicated the intergration and expression of foreign gene. The transformation frequency was 0.18%. In maize, the pollen grains of maize inbred line CA11 and Danyu No.13 were bombarded and used for pollination. R0 seeds were harvested and germinated in a nutrient solution containing Kanamycin 140 mg/L. GUS and Southern analysis confirmed the intergration and expression of the foreign gene. The transformation frequency was 0.05%￣0.21%.

关 键 词：基因枪 花粉 遗传转化 谷子 玉米 

分 类 号：S515.032[农业科学—作物学] S513.032