大鼠脑微血管内皮细胞的体外培养及鉴定  

Culture and Idendtification of Rat Brain Microvascular Endothelial Cells in vitro

作　　者：郭洋[1,2,3] 伊鹏霏[1,2] 吕钊君[2] 白慧云[2,4] 韦旭斌[1] 杜绍范[3] 穆祥[2] 

机构地区：[1]吉林大学畜牧兽医学院,长春130062 [2]北京农学院兽医学(中医药)北京市重点实验室,北京102206 [3]沈阳农业大学畜牧兽医学院,沈阳110866 [4]首都医科大学基础医学院,北京100068

出　　处：《沈阳农业大学学报》2010年第2期170-174,共5页Journal of Shenyang Agricultural University

基　　金：北京市自然科学基金重点项目(6061001);北京市教委"学术创新团队"项目(5090245)

摘　　要：探讨大鼠脑微血管内皮细胞的体外培养方法,为研究猪链球菌引起脑膜炎的致病机理打下基础。采用脑组织匀浆、二次过筛及酶消化技术分离大鼠脑微血管段,对分离的脑微血管内皮细胞进行了体外培养、形态学观察、免疫荧光鉴定及生长曲线的MTT法测定。结果表明:倒置显微镜下,细胞具有单层"铺路石样"排列的典型特征,免疫荧光检测第Ⅷ因子相关抗原呈阳性,成功建立了大鼠脑微血管内皮细胞的体外培养方法。The methods of culturing rat brain microvascular endothelial cells （BMECs） were studied to build up the basis for the studies of pathogenesis of meningitis caused by Streptococcus suis. We used the homogenization,filtration an denzyme digestion technique to isolate microvessels from rat brain. The isolated endothelial cells were cutured, observed morphologically and identified by immunofluorescence. We also determined the growth curve of BMECs by MTF assay. The cells looked like＂slabstone＂, a typical appearance of cultured endothelial cells under inverted microscope. Correlation antigens of VIB factor were positive by immunofluorescence. The results indicated that we have already succeeded in establishing the method of culturing rat brain microvascular endothelial cells（BMECs） in vitro.

关 键 词：大鼠 脑 微血管内皮细胞 培养 鉴定 

分 类 号：S858.28[农业科学—临床兽医学]