Sysmex UF-100全自动尿沉渣分析仪检测红细胞、白细胞的性能评价  

Valuation of Sysmex UF-100 fully automated urine sediment analyzer in detection of red blood cell and leukocyte

作　　者：王正蓉[1] 程树强[1] 夏曙华[1] 彭晓枫[1] 王琴[2] 

机构地区：[1]贵阳医学院检验系临床检验学教研室,贵州贵阳550004 [2]贵阳医学院医学检验系,贵州贵阳550004

出　　处：《遵义医学院学报》2010年第2期151-153,共3页Journal of Zunyi Medical University

摘　　要：目的评价Sysmex UF-100全自动尿沉渣分析仪检测尿红细胞（RBC）、白细胞（WBC）的性能。方法检测UF-100法的精密度,线性,携带污染率,并选择2~100/μl、100~500/μl、〉500/μl三个浓度范围的RBC、WBC标本各35例,分别用牛鲍氏细胞计数板和UF-100法进行计数并进行比对评价。结果 RBC和WBC精密度分别为3.8%和5.6%、线性相关系数（r）分别为0.997和0.999、携带污染率分别为0.14%和0.17%;UF-100与牛鲍氏计数板计数比较：RBC在〈500/μl浓度范围内无显著性差异（P〉0.05）,〉500/μl的浓度范围有显著性差异（P〈0.05）,WBC在2-100/μl浓度范围内无显著性差异（P〉0.05,）〉100/μl的浓度范围有显著性差异（P〈0.05）。结论 UF-100全自动尿沉渣分析仪检测RBC、WBC的精密度高,线性好,携带污染低率,但不能完全取代显微镜检查,结合尿干化学以及镜检联合过筛,可大大提高检测效率。Objective To evaluate the function of Sysmex UF-100 fully automated Urine sediment analyzer in detection of red blood cell（RBC） and leukocyte（WBC）.Methods To evaluate precision,linearity,carryover rate of the UF-100.select RBC,WBC samples in the range of 2~100/μl,100~500/μl, 500/μl,35 cases in each group,the samples were tested by the Neubauer Count Board and the UF-100 count method.The results of the two methods were compared.Results The precision of the RBC and WBC was 3.8% and 5.6% respectively,linear（r） were 0.997 and 0.999 respectively,the pollution carryover rate of 0.14% and 0.17%;Compare the results of the UF-100 and the Neubauer blood cell count board ： There were no significant difference in RBC 500/μl（P 0.05）,There were significant difference in RBC 500/μl（P 0.05）;WBC,no significant difference in the range of 2~100/μl（P0.05）, 100/μl were significant difference（P0.05）.Conclusions Use the UF-100 fully automated urine sediment analyzer to test RBC and WBC,its precision,linearity were well,and its carryover rate were low but it should not completely replace the microscopic examination,the UF-100t,he Dry chemistry and microscopic examination can greatly improve the efficiency of detection.

关 键 词：UF-100 性能评价 尿沉渣分析仪 

分 类 号：R392.12[医药卫生—免疫学]