Mg^2＋和Fe^3＋对日本血吸虫毛蚴的趋向作用观察及在“模拟钉螺”中的应用  

The effect of Mg^(2+) and Fe^(3+) on the distribution of Schistosoma japonicum miracidia and use of this information in simulated snails

作　　者：陈喜珪[1] 沈定文[1] 覃金红[1] 陈鹏程[1] 石磊[2] 刘洋[2] 卢恒聪[2] 何昌浩[2] 

机构地区：[1]咸宁学院基础医学院,湖北咸宁437100 [2]华中科技大学同济医学院病原生物学系

出　　处：《中国病原生物学杂志》2010年第7期529-530,501,共3页Journal of Pathogen Biology

基　　金：湖北省教育厅自然科学基金资助项目(No.D200628009)

摘　　要：目的观察Mg2+、Fe3+对日本血吸虫毛蚴的趋向作用及其在"模拟钉螺"中的应用。方法采用改良Ro-berts比较法。结果加入1.00mol/L的Mg2+溶液时,实验组和对照组中日本血吸虫毛蚴的数目比为93和21(P<0.01);Fe3+浓度为0.05、0.10、0.25、0.50和1.00mol/L时,实验组和对照组中日本血吸虫毛蚴的数目比分别是8l和29(P<0.01)、24和5(P<0.01)、225和39(P<0.01)、41和7(P<0.01)、52和17(P<0.01)。一定配比的明胶混合物释放出的Fe3+吸引日本血吸虫毛蚴的能力强于钉螺处理过的水。结论适宜浓度的Mg2+和Fe3+能显著影响日本血吸虫毛蚴的趋向运动,"模拟钉螺"吸引日本血吸虫毛蚴的能力强于钉螺处理过的水。Objective To observe the effect of Mg^2＋ and Fe^3＋ on the distribution of Schistosoma japonicum miracidia and use this information in simulated snails.Methods A modified form of Roberts＇ method of comparison was used.Results The ratio of S.japonicum miracidia for the experimental group and control group was 93：21（P0.01） when adding 1.00 mol/L Mg^2＋.The ratio of S.japonicum miracidia for the experimental group and control group was respectively 81：29（P0.01）,24：5（P0.01）,225：39（P0.01）,41：7（P0.01）,and 52：17（P0.01） when the concentration of Fe^3＋ was 0.05,0.10,0.25,0.50,and 1.00 mol/L.Fe^3＋ released from a gelatin mixture with a certain proportion of Mg^2＋ and Fe^3＋ had greater ability to attract S.japonicum miracidia than water conditioned by snails.Conclusion Mg^2＋ and Fe^3＋ significantly affected the distribution of S.japonicum miracidia.Simulated snails had greater ability to attract S.japonicum miracidia than water conditioned by snails.

关 键 词：血吸虫 日本 毛蚴 趋向作用 模拟钉螺 Mg2+和Fe3+ 

分 类 号：R383.24[医药卫生—医学寄生虫学]