2型糖尿病患者红细胞表面机械性质的原子力显微镜测量  

Mechanical properties of erythrocyte detected by atomic force microscope in type 2 diabetes mellitus

作　　者：金花[1] 陈纤纤[2] 赵宏霞[3] 伍时县[1] 蔡继业[1] 邢晓波[1] 黄训[1] 董世松[1] 黄飞程[1] 

机构地区：[1]暨南大学化学系,广州510632 [2]暨南大学第一附属医院 [3]广东工业大学轻工化工学院

出　　处：《中国糖尿病杂志》2010年第7期519-521,共3页Chinese Journal of Diabetes

基　　金：国家自然科学基金资助项目(60578025;30540420311)

摘　　要：目的探讨糖尿病患者与健康人红细胞形貌和纳米机械性质的差异。方法采用原子力显微镜(AFM)对10例2型糖尿病患者和8名健康志愿者的红细胞的形貌和机械性质进行纳米力学测量。结果 2型糖尿病患者的红细胞比健康人的硬度和黏附力都明显增大,细胞的高度、最高点与最低点的差值显著降低,而直径增大。结论 AFM为单细胞水平上表征红细胞的病理特征检查提供了有力工具。Objective To measure the difference in morphology and nanomechanical properties（NP, including elasticity and adhesion）of erythrocytes between groups of type 2 diabetic patients（T2DM,n= 10） and healthy volunteers （HV, n = 8）. Methods Erythrocytes were detected by atomic force microscope （AFM）. Results Erythrocyte nanostiffness and adhesion were higher in T2DM than in HV. There was remarkable changes in cell mean height, value of cell peak to valley, and diameter of red blood cell. Conclusions AFM could provide pathological information of erythrocytes at single cell level and AFM could be a powerful tool in disease diagnosis.

关 键 词：糖尿病 2型 红细胞 原子力显微镜 

分 类 号：R587.1[医药卫生—内分泌]