Artificial induction of mito-gynogenetic diploids in large yellow croaker(Pseudosciaena crocea) by hydrostatic pressure  

作　　者：蔡明夷 吴清明 刘贤德 姚翠鸾 陈庆凯 王志勇 

机构地区：[1]Key Laboratory of Science and Technology for Aquaculture and Food Safety,Fisheries College,Jimei University [2]Fishery Technical Extension Station of Ningde City

出　　处：《Chinese Journal of Oceanology and Limnology》2010年第4期713-719,共7页中国海洋湖沼学报（英文版）

基　　金：Supported by the National High Technology Research and Development Program of China(863 Program)(No.2006AA10A405);the Foundation for Innovative Research Team of Jimei University(No.2006A001),the Science Foundation of Jimei University(No.ZQ2006037)

摘　　要：The present study investigated conditions for inducing mito-gynogenetic(endomitosis) diploids by hydrostatic pressure in the large yellow croaker Pseudosciaena crocea.In haploid control groups,the development of eggs was activated with ultraviolet radiated semen.All fry presented typical haploid syndrome in the haploid control groups,and were verified as haploids using cytometry.After hydrostatic pressure treatment,morphologically normal fry reappeared at different frequencies according to the intensity and time of pressure shock.Fry with normal appearance in the pressure treated groups were verified as gynogenetic double haploids(GDHs),containing only one allele from the female parent at all four diagnostic microsatellite loci.For a fixed duration of 3 min,the optimal intensity of blocking the first mitosis was determined to be 40 Mpa,which was similar to that of blocking the second meiosis.There was a "window" of starting time,from 36.1 min to 38.1 min post-insemination at 25.0±1.0°C,within which the production of GDHs was not significantly different.Maximum production of morphologically normal fries,9.36%±2.97% of developed eggs,was found when the eggs were shocked with hydrostatic pressure at 40 Mpa for 3 min,starting from 38.1 min post insemination at 25.0±1.0°C.The present study investigated conditions for inducing mito-gynogenetic（endomitosis） diploids by hydrostatic pressure in the large yellow croaker Pseudosciaena crocea.In haploid control groups,the development of eggs was activated with ultraviolet radiated semen.All fry presented typical haploid syndrome in the haploid control groups,and were verified as haploids using cytometry.After hydrostatic pressure treatment,morphologically normal fry reappeared at different frequencies according to the intensity and time of pressure shock.Fry with normal appearance in the pressure treated groups were verified as gynogenetic double haploids（GDHs）,containing only one allele from the female parent at all four diagnostic microsatellite loci.For a fixed duration of 3 min,the optimal intensity of blocking the first mitosis was determined to be 40 Mpa,which was similar to that of blocking the second meiosis.There was a ＂window＂ of starting time,from 36.1 min to 38.1 min post-insemination at 25.0±1.0°C,within which the production of GDHs was not significantly different.Maximum production of morphologically normal fries,9.36%±2.97% of developed eggs,was found when the eggs were shocked with hydrostatic pressure at 40 Mpa for 3 min,starting from 38.1 min post insemination at 25.0±1.0°C.

关 键 词：GYNOGENESIS double haploid large yellow croaker Pseudosciaena crocea MICROSATELLITE 

分 类 号：S917.4[农业科学—水产科学]