Molecular phylogeny of oligotrich genera Omegastrombidium and Novistrombidium(Protozoa,Ciliophora) for the systematical relationships within Family Strombidiidae  

作　　者：张倩倩 伊珍珍 徐大鹏 AL-RASHEID Khaled A.S. 龚骏 宋微波 

机构地区：[1]Laboratory of Protozoology,Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education,College of Life Science,South China Normal University [2]Laboratory of Protozoology,Institute of Evolution and Marine Biodiversity,Ocean University of China [3]Biology Department,North Carolina Central University [4]Zoology Department,King Saud University

出　　处：《Chinese Journal of Oceanology and Limnology》2010年第4期769-777,共9页中国海洋湖沼学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China(Nos.30870264and40976099);the Centre of Excellence in Biodiversity,King Saud University

摘　　要：The phylogeny of the oligotrich ciliates is currently a hot debate despite the availability of both morphological and molecular data.In the present paper,further small subunit rRNA(SS rRNA) genes were analyzed from the Genera Omegastrombidium and Novistrombidium,as well as from Strombidium,and combined with three new SS rRNA sequences from Strombidium basimorphum,S.sulcatum population QD-1,and Novistrombidium testaceum population GD.The phylogenetic positions of these organisms were inferred using Bayesian inference,Maximum Likelihood,and Maximum Parsimony methods.The main results are:(1) the SS rRNA gene sequence analyses match the recent findings about the molecular evolution of oligotrichs,indicating that the family Strombidiidae is paraphyletic;(2) the Genus Omegastrombidium is separated from the Genus Strombidium,as shown in recent cladistic analyses;(3) morphospecies in Genus Novistrombidium,based on similarity of somatic ciliature,are separated from each other in all topological trees,indicating that this genus could be a paraphyletic group;(4) the molecular data indicate a possibility of paraphyly for the genus Strombidium;and(5) the similarities of the SS rRNA gene of specimens identified as S.sulcatum and S.inclinatum are 99.8%-100%.However,present knowledge on the oligotrichs sensu stricto is still insufficient and further studies based on both molecular and other technologies are required.The phylogeny of the oligotrich ciliates is currently a hot debate despite the availability of both morphological and molecular data.In the present paper,further small subunit rRNA（SS rRNA） genes were analyzed from the Genera Omegastrombidium and Novistrombidium,as well as from Strombidium,and combined with three new SS rRNA sequences from Strombidium basimorphum,S.sulcatum population QD-1,and Novistrombidium testaceum population GD.The phylogenetic positions of these organisms were inferred using Bayesian inference,Maximum Likelihood,and Maximum Parsimony methods.The main results are：（1） the SS rRNA gene sequence analyses match the recent findings about the molecular evolution of oligotrichs,indicating that the family Strombidiidae is paraphyletic; （2） the Genus Omegastrombidium is separated from the Genus Strombidium,as shown in recent cladistic analyses; （3） morphospecies in Genus Novistrombidium,based on similarity of somatic ciliature,are separated from each other in all topological trees,indicating that this genus could be a paraphyletic group; （4） the molecular data indicate a possibility of paraphyly for the genus Strombidium; and（5） the similarities of the SS rRNA gene of specimens identified as S.sulcatum and S.inclinatum are 99.8%-100%.However,present knowledge on the oligotrichs sensu stricto is still insufficient and further studies based on both molecular and other technologies are required.

关 键 词：Omegastrombidium Novistrombidium Strombidium SS rRNA PHYLOGENY 

分 类 号：Q951[生物学—动物学]