A genetic linkage map of marine shrimp Penaeus (Fenneropenaeus) chinensis based on AFLP, SSR, and RAPD markers  

作　　者：刘博 王清印 李健 刘萍 何玉英 

机构地区：[1]College of Marine Life Sciences,Ocean University of China [2]Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences

出　　处：《Chinese Journal of Oceanology and Limnology》2010年第4期815-825,共11页中国海洋湖沼学报（英文版）

基　　金：Supported by National Key Technology R&D Program(No.2006BAD01A13);National Special Research Fund for Nonprofit Sector(Agriculture)(No.nyhyzx07-042);the Earmarked Fund for Modern Agro-industry Technology Research System(No.nycytx-46)

摘　　要：The Chinese shrimp Penaeus (Fenneropaeneus) chinensis is an important species in marine fishery and aquaculture in China. A female Chinese shrimp Penaeus (Fenneropaeneus) chinensis was captured from west coast of the Korean peninsula and mated with a "Yellow Sea No. 1" male to produce the first filial generation (F1) 100 F2 full-sib progeny from brother-sister crosses between F1 families was used for the mapping study. A genetic linkage map of the Chinese shrimp was constructed, based on 354 markers, including 300 amplified fragment length polymorphism (AFLP) markers, 42 microsatellite (SSR) markers, and 12 randomly amplified polymorphism (RAPD) markers. Forty-seven linkage groups (LGs) were identified. The total map length was 4 580.5 cM, with an average spacing of 11.3 cM, covering 75.8% of the estimated genome size. The construction of this genetic linkage map was part of a genetic breeding program. This linkage map will contribute to the discovery of genes and quantitative trait loci (QTLs) in Chinese shrimp.The Chinese shrimp Penaeus （Fenneropaeneus） chinensis is an important species in marine fishery and aquaculture in China. A female Chinese shrimp Penaeus （Fenneropaeneus） chinensis was captured from west coast of the Korean peninsula and mated with a ＂Yellow Sea No. 1＂ male to produce the first filial generation （F1） 100 F2 full-sib progeny from brother-sister crosses between F1 families was used for the mapping study. A genetic linkage map of the Chinese shrimp was constructed, based on 354 markers, including 300 amplified fragment length polymorphism （AFLP） markers, 42 microsatellite （SSR） markers, and 12 randomly amplified polymorphism （RAPD） markers. Forty-seven linkage groups （LGs） were identified. The total map length was 4 580.5 cM, with an average spacing of 11.3 cM, covering 75.8% of the estimated genome size. The construction of this genetic linkage map was part of a genetic breeding program. This linkage map will contribute to the discovery of genes and quantitative trait loci （QTLs） in Chinese shrimp.

关 键 词：AFLP linkage map Penaeus （Fenneropenaeus） chinensis RAPD SSR 

分 类 号：S917.4[农业科学—水产科学]