Recover vigorous cells of Magnetospirillum magneticum AMB-1 by capillary magnetic separation  

作　　者：李金华 葛欣 张小葵 陈冠军 潘永信 

机构地区：[1]State Key Laboratory of Microbial Technology,Shandong University [2]Biogeomagnetism Group,Paleomagnetism and Geochronology Laboratory(SKL-LE),Institute of Geology and Geophysics,Chinese Academy of Sciences [3]Marine College,Shandong University at Weihai

出　　处：《Chinese Journal of Oceanology and Limnology》2010年第4期826-831,共6页中国海洋湖沼学报（英文版）

基　　金：Supported by the Natural Science Foundation of Shandong Province,China(No.2006ZRB01973);the National Natural Science Foundation of China(Nos.40821091,40325011)

摘　　要：Cultivable magnetotactic bacteria(MTB) in laboratory can provide sufficient samples for molecular microbiological and magnetic studies.However,a cold-stored MTB strain,such as Magnetospirillum magneticum AMB-1,often loses its ability to synthesize magnetosomes and consequently fails to sense the external magnetic field.It is therefore important to quickly recover vigorous bacteria cells that highly capable of magnetosome producing.In this study,a modified capillary magnetic separation system was designed to recover a deteriorating strain of Magnetospirillum magneticum AMB-1 that long-term cold-stored in a refrigerator.The results show that all cells obtained after a 3-cycle treatment were vigorous and had the ability to produce magnetosomes.Moreover,the 3rd-cycle recovered cells were able to form more magnetosome crystals.Compared with the colony formation method,this new method is time-saving,easily operated,and more efficient for recovering vigorous MTB cells.Cultivable magnetotactic bacteria（MTB） in laboratory can provide sufficient samples for molecular microbiological and magnetic studies.However,a cold-stored MTB strain,such as Magnetospirillum magneticum AMB-1,often loses its ability to synthesize magnetosomes and consequently fails to sense the external magnetic field.It is therefore important to quickly recover vigorous bacteria cells that highly capable of magnetosome producing.In this study,a modified capillary magnetic separation system was designed to recover a deteriorating strain of Magnetospirillum magneticum AMB-1 that long-term cold-stored in a refrigerator.The results show that all cells obtained after a 3-cycle treatment were vigorous and had the ability to produce magnetosomes.Moreover,the 3^rd-cycle recovered cells were able to form more magnetosome crystals.Compared with the colony formation method,this new method is time-saving,easily operated,and more efficient for recovering vigorous MTB cells.

关 键 词：magnetotactic bacteria Magnetospirillum magneticum AMB-1 capillary magnetic separation strain recovery 

分 类 号：Q938[生物学—微生物学]