以虫草素为配体的钌多吡啶(Ⅱ)配合物的抗肿瘤活性研究  

Studies on the cytotoxic properties of ruthenium(Ⅱ) complexes coordinated by cordycepin

作　　者：孙冬冬[1] 梅文杰[1,2] 刘杰[2] 黄东纬[1] 杨小辛[2] 郑文杰[2] 

机构地区：[1]广东药学院药科学院,广东广州510006 [2]暨南大学生命科学学院,广东广州510632

出　　处：《广东药学院学报》2010年第3期264-268,共5页Academic Journal of Guangdong College of Pharmacy

基　　金：广东省自然科学基金博士启动基金(04300624);广东省科技计划项目资助(2007B031513004)

摘　　要：目的研究虫草素为配体的钌多吡啶(Ⅱ)配合物的抗肿瘤活性。方法以虫草素(3′-脱氧腺苷)为配体,合成了3种钌(Ⅱ)配合物[Ru(L)2(DOA)]Cl2(L=bpy,1;phen,2;dmbpy,3;DOA=3′-脱氧腺苷),采用MTT法和流式细胞术分析体外抗肿瘤活性。结果 MTT法结果表明配合物1,2和3对皮肤癌细胞A375生长表现出明显的抑制作用,且其抗肿瘤活性优于配体虫草素;流式细胞分析结果表明,配合物1是通过诱导肿瘤细胞凋亡抑制肿瘤细胞生长。结论以虫草素为配体的钌多吡啶配合物具有一定的抗肿瘤活性。ObjectiveTo Study on the cytotoxic properties of ruthenium（Ⅱ） complexes coordinated by cordycepin. Methods Three ruthenium（Ⅱ） complexes coordinated by cordycepin [Ru（L）2（DOA）]Cl2（L=bpy,1;phen,2;dmbpy,3;DOA= cordycepin） have been synthesized and their inhibitory activity against skin cancer were evaluated by MTT assay and flow cytometry.Results MTT assay results showed that all of these complexes exhibit higher inhibitory activity against growth of skin cancer cells A375 than their mother type compound cordycepin.Further studies by Flow CytoMeter（FCM） on 1 showed that it can induce apoptosis of skin cancer cells A375 to inhibit their growth.Conclusion The ruthenium（Ⅱ） complexes have some anti-tumor activity.

关 键 词：虫草素 钌(Ⅱ)多吡啶配合物 抗肿瘤活性 MTT分析 流式细胞术 

分 类 号：R91[医药卫生—药学] R96