丹参总酚酸与三七总皂苷合用4种丹酚酸成分在大鼠体内的药动学  

Pharmacokinetics of the Combination of Salvianolic Acids and Panax Notoginseng Saponins in Rat Plasma

作　　者：姚燕[1] 陈晓辉[1] 果德安[2] 张金强[1] 陈昕[2] 毕开顺[1] 

机构地区：[1]沈阳药科大学药学院,沈阳110016 [2]中国科学院上海药物研究所中药现代化研究中心,上海201203

出　　处：《中国药学杂志》2010年第15期1163-1167,共5页Chinese Pharmaceutical Journal

摘　　要：目的建立丹参总酚酸与三七总皂苷合用(5:1)灌胃给药后大鼠血浆中原儿茶醛、迷迭香酸、紫草酸、丹酚酸B含量的测定方法,并对其进行药动学研究。方法采用HPLC-UV方法测定,色谱柱:ZorbaxSB-C18column(4.6mm×250mm,5μm),流速:1mL.min-1,柱温:30℃,检测波长:280nm,以乙腈和体积分数为0.026%的磷酸水溶液梯度洗脱,血浆样品以体积分数为10%的盐酸酸化,而后用乙酸乙酯萃取2次,并根据测定结果求算药动学参数。结果 4种成分的日内与日间RSD均小于15%,提取回收率均大于70%,线性关系良好。原儿茶醛在10.0min达峰,ρmax为6.466mg.L-1,AUC0-∞值为82.1mg.min.L-1,迷迭香酸在11.7min达峰,ρmax为10.50mg.L-1,AUC0-∞值为564.8mg.min.L-1,紫草酸在20.8min达峰,ρmax为5.950mg.L-1,AUC0-∞值为1123mg.min.L-1,丹酚酸B在8.3min达峰,ρmax为48.12mg.L-1,AUC0-∞值为4026mg.min.L-1。结论该法适用于原儿茶醛、迷迭香酸、紫草酸和丹酚酸B的血药浓度测定与药动学研究。OBJECTIVE To determine four phenolic acids （procatechualdehyde,rosmarinic acid,lithospermic acid,salvianolic acid B） in rat plasma after oral administration of salvianolic acids and Panax notoginseng saponins （5：1）,and to evaluate its pharmacokinetics.METHODS A high-performance liquid chromatography （HPLC） method was established for the simultaneous determination of four phenolic acids.Chromatographic separation was achieved on a Zorbax SB-C18 column （4.6 mm×250 mm,5 μm） at 30 ℃,with a linear gradient elution of acetonitrile and 0.026% aqueous phosphoricacid （v/v）,at the flow rate of 1 mL·min-1.The eluent was detected at 280 nm for the four phenolic acids and internal standard.The plasma sample was extracted with ethyl acetate twice after acidification by 10% hydrochloric acid.The pharmacokinetic parameters were obtained.RESULTS The within-and between-day variation for the four phenolic acids in plasma were below 15% and the extraction recovery was above 70%.The pharmacokinetic parameters for the four phenolic acids were as followes：tmax,ρmax and AUC0-∞ were 10.0 min,6.466 mg·L-1 and 82.1 mg·min·L-1 for procatechualdehyde;11.7 min,10.50 mg·L-1 and 564.8 mg·min·L-1 for rosmarinic acid,respectively;20.8 min,5.950 mg·L-1 and 1 123 mg·min·L-1 for lithospermic acid respectively;8.3 min,48.12 mg·L-1 and 4 026 mg·min·L-1 for salvianolic acid B.CONCLUSION The method is simple,rapid and sensitive for the determination of four compounds in rat plasma and the study of pharmacokinetics.

关 键 词：高效液相色谱法 丹参总酚酸 三七总皂苷 药动学 

分 类 号：R969.1[医药卫生—药理学]