HPLC法测定保健食品中左旋肉碱含量的研究  

Detemination of L-camitine content in health food by HPLC

作　　者：甘宾宾[1] 黎少豪[1] 

机构地区：[1]广西壮族自治区疾病预防控制中心,南宁530021

出　　处：《中国卫生检验杂志》2010年第7期1688-1689,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的:建立高效液相色谱法(HPLC)测定保健食品中左旋肉碱含量的简便方法。方法:样品用水超声提取,色谱柱:CAPCELL PAK CR(4.6×150 mm,5μm);流动相:20 mmol乙酸铵+乙腈(加0.1%甲酸)(25+75,v/v);流量:1.0 ml/min,检测波长:210 nm。结果:左旋肉碱在1.2μg～105μg范围内呈良好线性,相关系数为0.999987,检出限为0.08μg,回收率97.7%～101%,RSD1.10%。结论:该方法操作简便、快速、结果准确、可靠,可作为保健食品中左旋肉碱的质量控制监测。Objective：To establish an method for determination of L-camitine conten in health food hy HPLC.Methods： The samples were extracted by ultrasonic water.The separarion was performed on a CAPCELLPAKCR column（4.6×150 mm,5 μm）;the mobile phase consisted of 20 mmol Ammonium acetate＋Acetonitrile（0.1% Formic acid）（25＋75,v/v）;the flow rate was 1.0 ml/min;the detection wavelength was 210 nm.Results： The L-camitine was shown to be linear over the range from 1.2 μg～105 μg（r=0.999987）.The limit of detection was 0.08 μg.The recovery was 97.7% ～101%,RSD 1.10%.Conclusion： This method is simple,accurate and reliable,which can be used for the quality control of L-camitine in health food.

关 键 词：HPLC 左旋肉碱 研究 

分 类 号：O657.72[理学—分析化学]