一种新型检测乙型肝炎病毒核酸方法的建立  

Build of a new method for detection of HBV-DNA

作　　者：张永乐[1] 任超学[2] 钟华燕[1] 

机构地区：[1]杭州市第六人民医院,杭州310014 [2]西安体育学院,西安710068

出　　处：《中国卫生检验杂志》2010年第7期1709-1710,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的:建立一种新型快速检测乙型肝炎病毒核酸(HBVDNA)的方法。方法:根据乙肝病毒S保守区设计3对引物进行乙肝病毒DNA切口酶核酸恒温扩增,产物在密封装置中进行试纸条的显色反应,根据显色深浅进行定量。结果:恒温扩增试纸条技术检测血清HBVDNA的特异性100%,敏感度达到100拷贝/毫升。结论:恒温扩增试纸条法检测时间短,不受接收标本时间限制随时可以检测,特异性强,敏感度高,不需昂贵的定量PCR检测仪,便于更多医院检验科的开展。Objective：The establishment of a new rapid detection method of HBVDNA.Methods：According to conservative hepatitis B virus S designed three pairs of primers for hepatitis B virus DNA incision constant temperature of nucleic acid amplification,the product of the sealing device in a PCR test paper was studied.Results：Isothermal amplification test paper to detect the specificity of 100% of serum HBVDNA sensitivity of 100 copies/ml.Conclusion：Thermostat PCR amplification test paper in HBVDNA detection of hepatitis B virus sensitivity and specificity and fluorescence quantitative PCR detection of exactly the same.PCR amplification test paper temperature detected a short time,without time limit to receive samples can be detected at any time,without expensive for a more quantitative PCR testing for more hospitals in the laboratory.

关 键 词：乙型肝炎病毒 恒温扩增 试纸条 荧光定量PCR 

分 类 号：R373.2[医药卫生—病原生物学]