新疆哈萨克族脂蛋白脂酶基因Hind Ⅲ、S447X多态性与代谢综合征的相关研究  

Association of lipoprotein lipase gene Hind Ⅲ and S447X polymorphisms with metabolic syndrome in Kazakh of Xinjiang

作　　者：杨志明[1] 郭淑霞[1] 张景玉[1] 郭恒[1] 马儒林[1] 徐上知[1] 芮东升[1] 

机构地区：[1]新疆石河于大学医学院预防医学系,石河子832002

出　　处：《中华内分泌代谢杂志》2010年第7期555-559,共5页Chinese Journal of Endocrinology and Metabolism

基　　金：＂十一五＂国家科技支撑计划（2009BAI82B04）;新疆生产建设兵团国际科技合作项目（2009YD33）

摘　　要：目的 探讨脂蛋白脂酶基因Hind Ⅲ、S447X多态性与代谢综合征（MS）的关系.方法 应用聚合酶链反应-限制性片段长度多态法检测401例研究对象（包括200例代谢综合征患者和201名对照者）的脂蛋白脂酶Hind Ⅲ、S447X基因型.结果 （1）腰围、臀围、腰臀比、体重指数（BMI）、收缩压、舒张压、总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）、空腹血糖（FPG）在MS组与对照组之间差别均有统计学意义（均P〈0.05）.（2）代谢综合征组H＋H＋基因型、H＋等位基因、SS基因型和S等位基因频率显著高于对照组（H＋H＋基因型：66.5%对54.2%,P=0.012;H＋等位基因：78.0%对71.4%,P=0.031;SS基因型：89.5%对77.1%,P=0.001;S等位基因：94.5%对87.56%,P=O.001）.（3）H＋H_/H-H-基因型携带者腰围、臀围、腰臀比、BMI、收缩压、舒张压、TG、LDL-C、FPG水平较H＋H＋基因型携带者低,HDL-C水平高于H＋H＋基因型携带者,差别均有统计学意义（均P〈0.05）.SX/XX基因型携带者腰围、臀围、腰臀比、BMI、收缩压、舒张压、TC、TG、FPG水平较SS基因型携带者低,HDL-C水平高于SS基因型携带者,差别均有统计学意义（均P〈0.05）.（4）多因素Logistic回归分析显示吸烟、饮酒、SS基因型是MS的危险因素（OR值分别为4.289、2.268、2.597）.（5）不同因素间的交互作用结果显示,吸烟者携带SS基因型的患病风险是不吸烟者携带SX/XX基因型的3.996倍.结论 脂蛋白脂酶基因S447X多态性与哈萨克族MS的危险性相关,其中SS基因型、S等位基因可能是代谢综合征的遗传危险因素,而且H＋H-/H-H-和SX/XX基因型对血脂、血压产生有益的影响.SS基因型与吸烟可能存在交互作用,二者之间有相加作用.Objective To investigate the association of lipoprotein lipase gene Hind Ⅲ and S447X polymorphisms with metabolic syndrome. Methods PCR-RFLP was used to detect lipoprotein lipase Hind Ⅲ and S447X genotypes in 401 subjects（including 201 controls, 200 metabolic syndrome patients）. Results （ 1 ） The levels of waist circumference （ WC ） , hip circumference （ HC ） , waist-to-hip ratio （ WHR ） , body mass index （BMI）, systolic blood pressure （SBP）, diastolic blood pressure （ DBP） , total cholesterol （ TC） , triglyceride （TG）, high-density lipoprotein-cholesterol （HDL-C）, low-density lipoprotein-cholesterol （LDL-C）, and fasting plasma glucose （FPG） were significantly different between metabolic syndrome group and control group （all P〈 0.05）. （2）The frequencies of H＋H＋ genotype,H＋allele,SS genotype, and S allele for metabolic syndrome were all significantly higher than those for controls（ H＋H＋ genotype：66. 5% vs 54.2% ,P=0.012; H＋ allele：78.0% vs 71.4%, P=0.031;SS genotype：89.5% vs 77. 1% , P = 0.001; Sallel：94.5% vs 87. 56% , P = 0.001）. （3） The levels of WC, HC, WHR, BMI, SBP, DBP, TG, LDL-C, and FPG in H ＋ H-/H-H- genotype were significantly lower than those in H＋H＋ genotype, HDL-C was significantly higher than that in H＋H＋ genotype （ all P〈0. 05）. The levels of WC, HC, WHR, BMI, SBP, DBP, TC, TG, and FPG in SX/XX genotype were significantly lower than those in SS genotype, HDL-C was significantly higher than that in SS genotype （ all P〈 0.05）. （4）Multi-way logistic regression analysis suggested that risk factors for metabolic syndrome were smoking, drinking, and SS genotype （OR value was 4.289,2.268, and 2. 597, respectively ）. （5） Result of interaction analysis among different factors indicated that the risk for metabolic syndrome in smoker with SS genotype was 3. 996 times of non-smokers with SX/XX genotype. Conclusions The lipoprotein lipase gene S447X polymorphism is associated with meta

关 键 词：哈萨克族 代谢综合征 脂蛋白脂酶 基因多态性 

分 类 号：R589[医药卫生—内分泌]