胰岛素抵抗大鼠肝脏组织PERK和p-PERK的表达及意义  

Expressions of PERK and p-PERK in the Livers of Insulin-Resistant Rats and Their Significance

作　　者：林志楠[1] 罗红飞[1] 都健[1] 裴丽娜[1] 刘佳[1] 郭宁宁[1] 张克莹[1] 

机构地区：[1]中国医科大学附属第一医院内分泌科,沈阳110001

出　　处：《中国医科大学学报》2010年第7期529-531,538,共4页Journal of China Medical University

基　　金：辽宁省科学技术计划项目(2009225029)

摘　　要：目的探讨胰岛素抵抗大鼠肝脏组织PKR样内质网调节激酶(PERK)及磷酸化PERK(p-PERK)的表达及意义。方法 SPF级雄性Wistar大鼠30只,随机分为正常饮食组(NC组)和高脂饮食组(HF组),每组15只。喂养10周后,以高血浆胰岛素-正常血糖钳夹技术评估胰岛素抵抗大鼠模型。应用Westernblot方法检测肝脏组织中PERK和p-PERK蛋白的表达。结果与NC组相比,HF组60～120min的平均葡萄糖输注率(GIR60～120)明显低于NC组(0.90±0.15mg·kg-1·min-1vs4.97±0.68mg·kg-1·min-1,P<0.01)。与NC组相比,HF组大鼠肝脏p-PERK表达明显升高(192.89±25.16vs63.04±15.61,P<0.05),p-PERK/PERK也明显升高(0.99±0.12vs0.33±0.08,P<0.05)。结论胰岛素抵抗大鼠肝脏组织中p-PERK蛋白及p-PERK/PERK升高,提示内质网应激可能参与胰岛素抵抗的发生。Objective To investigate the expressions of PKR-like endoplasmic reticulum kinase（PERK）and phosphorylated PERK（pPERK）in the livers of insulin-resistant rats and their significance.Methods Thirty specific pathogen-free male Wistar rats were randomly divided into normal diet group（NC group）and high-fat diet group（HF group）（n=15 each group）.Insulin-resistant rat models were assessed by hyperinsulinemic-euglycemic clamp technique after fed with high-fat diet for 10 weeks.The expressions of PERK and p-PERK protein in the livers were detected by Western blot.Results Compared with NC group,the glucose infusion rate（GIR60～120）was significantly lower（0.90±0.15 vs 4.97±0.68 mg·kg-1·min-1,P〈0.01）and the level of p-PERK in the liver（192.89±25.16 vs 63.04±15.61,P〈0.05） and p-PERK/PERK（0.99±0.12 vs 0.33±0.08,P〈0.05）were significantly higher in HF group.Conclusion The increase of p-PERK protein and p-PERK/PERK in the livers of insulin-resistant rats indicate that endoplasmic reticulum stress may be involved in insulin resistance.

关 键 词：胰岛素抵抗 内质网应激 大鼠 肝脏组织 磷酸化PKR样内质网调节激酶 

分 类 号：R587.1[医药卫生—内分泌]