卵泡液中白细胞介素2、白细胞介素12、白细胞介素18与体外受精-胚胎移植妊娠结局的相关性  

Correlation between Follicular Fluid Interleukin-2,Interleukin-12 and Interleukin-18 and Outcome of in Vitro Fertilization Embryo Transfer

作　　者：王秀霞[1] 王天任[1] 何丽霞[1] 孔琳[1] 徐小延[1] 杨大磊[1] 

机构地区：[1]中国医科大学附属盛京医院妇产科,沈阳110004

出　　处：《中国医科大学学报》2010年第8期643-645,共3页Journal of China Medical University

基　　金：辽宁省科学技术基金资助项目(2007118)

摘　　要：目的探讨卵泡液中细胞因子白细胞介素2(IL-2)、IL-12、IL-18与体外受精-胚胎移植妊娠结局的关系。方法接受体外受精-胚胎移植助孕治疗的妇女117例,其中妊娠妇女59例,未妊娠妇女58例,应用酶联免疫吸附试验检测卵泡液中IL-2、IL-12、IL-18的浓度。结果妊娠组卵泡液中IL-2的浓度为(36.73±18.53)pg/ml、IL-12的浓度为(0.52±0.88)pg/ml、IL-18的浓度为(76.10±69.06)pg/ml;未妊娠组卵泡液中IL-2的浓度为(40.89±22.83)pg/ml、IL-12的浓度为(1.59±1.58)pg/ml、IL-18的浓度为(131.80±162.35)pg/ml。未妊娠组IL-12和IL-18浓度均明显高于妊娠组(P<0.05),未妊娠组IL-2浓度虽较妊娠组升高,但差异无统计学意义(P>0.05)。结论 IL-12、IL-18在接受体外受精-胚胎移植助孕治疗的非妊娠妇女中明显升高,考虑与体外受精-胚胎移植妊娠结局负相关。Objective To investigate the relationship between the concentration of interleukin-2（IL-2）,interleukin-12（IL-12）and interleukin-18（IL-18）in follicular fluid and the clinical pregnancy rate in in vitro fertilization embryo transfer（IVF-ET）treatment.Methods Enzyme-linked immunosorbent assay（ELISA）was used to analyze the concentration of IL-2,IL-12 and IL-18 in follicular fluid.The IVF treatment outcome was closely followed up.The relationship between the concentration and the outcome was discussed by statistical analysis.Results Patients without successful IVF-ET outcome had concentration of increased IL-2（40.89±22.83 pg/ml,P〈0.05）,concentration of IL-12（1.59±1.58 pg/ml,P〈0.05）and IL-18（131.8±162.35 pg/ml,P〈0.05）compared with patients who achieved clinical pregnancy IL2（36.73±18.53 pg/ml）,IL-12（0.52±0.88 pg/ml）and IL-18（76.10±69.06 pg/ml）.Conclusion Women received IVF treatment with higher concentrations of IL-12 and IL-18 in mature follicular fluid are more difficult to get pregnancy.Altered concentrations of IL-12 and IL18 in follicular fluid could therefore influence fertility in IVF treatment outcome.

关 键 词：体外受精-胚胎移植 妊娠 卵泡液 细胞因子 白细胞介素2 白细胞介素12 白细胞介素18 

分 类 号：R715.2[医药卫生—妇产科学]