浙江省蜱标本中斑点热群立克次体rOmpA和gltA基因检测  

Detection of rOmpA and gltA genes of spotted fever group rickettsiae from tick specimens in Zhejiang province

作　　者：姜理平[1] 孟真[1] 崔清荣 童卫胜[3] 凌峰[1] 王臻[1] 

机构地区：[1]浙江省疾病预防控制中心微生物所,杭州310051 [2]天台县疾病预防控制中心 [3]临安市疾病预防控制中心

出　　处：《中国媒介生物学及控制杂志》2010年第4期350-352,共3页Chinese Journal of Vector Biology and Control

摘　　要：目的了解浙江省部分山区蜱中感染斑点热群立克次体的状况,探讨以外膜蛋白A(rOmpA)和柠檬酸合成酶基因(gltA)为靶基因的PCR方法可行性。方法利用PCR方法,检测天台县左溪镇和临安县西天目地区共46组蜱类标本中rOmpA和gltA基因特异片段。对所检测到的阳性结果进行克隆与序列测定,并进行聚类分析。结果从46组蜱标本中检测发现2组长角血蜱中斑点热群立克次体的rOmpA和gltA基因片段均为阳性,核酸序列基本一致,但推测斑点热群立克次体种的进化位置存在差异。结论浙江省部分山区存在蜱中感染斑点热群立克次体的状况。利用rOmpA和gltA基因能从标本中检测到斑点热群立克次体,但从2个结果推测立克次体的分类位置存在差异。Objective To identify the prevalence of ticks infected with spotted fever group rickettsiae in mountain areas of Zhejiang province,and to explore the feasibility of the PCR approach using rickettsial outer membrane protein A（rOmpA） and citrate synthase（gltA） gene as the target genes.Methods The rOmpA and gltA gene specific fragments were assessed using the PCR method from 46 tick specimens in Tiantai Zuoxi county and Lin＇an Xitianmu region.The positive samples were cloned and sequenced followed by cluster analysis.Results The rOmpA and gltA gene specific fragments of spotted fever group rickettsiae were detected from 2 Haemaphysalis longicornis of the 46 specimens,with similar nucleic acid sequences.However,the evolutionary positions of the spotted fever group rickettsia species were presumably different between the two.Conclusion The ticks infected with spotted fever group rickettsiae were found in some mountain areas of Zhejiang province.Spotted fever group rickettsiae were detected from the tick specimens using the rOmpA and gltA genes,though the deduced diversification of rickettsial species of the two samples differed.

关 键 词：斑点热群立克次体 外膜蛋白A基因 柠檬酸合成酶基因 聚合酶链反应 蜱 

分 类 号：R376[医药卫生—病原生物学]