氯沙坦对日本血吸虫病肝纤维化小鼠αSMA及TGFβ1的影响  

Effect of Losartan on Expression of AT1R mRNA and TGF-β_1 mRNA in Hepatic Fibrosis of Mice Infected with Schistosoma japonicum

作　　者：黄海燕[1,2] 艾国[1] 焦云桃[1] 李兰[1] 郭威[1] 黄加权[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院感染科,湖北武汉430030 [2]咸宁市第一人民医院病理科,湖北咸宁437000

出　　处：《中华医院感染学杂志》2010年第15期2192-2195,共4页Chinese Journal of Nosocomiology

基　　金：湖北省血吸虫病防治项目(XF06c28;XF2008-11)

摘　　要：目的探讨氯沙坦影响日本血吸虫病鼠早期肝纤维化的部分相关机制。方法将36只昆明小鼠随机分为正常对照组,感染对照组及氯沙坦组,采用日本血吸虫尾蚴35条/只鼠攻击感染小鼠建立血吸虫性肝纤维化模型,氯沙坦10 mg/(kg.d),灌胃,1次/d,连续6周,HE染色观察肝组织病理学改变;放射免疫法检测血清中透明脂酸(HA)含量,SP法检测α平滑机动蛋白(SMA),金属蛋白酸因子(TIMP)-1在肝组织中的表达;实时荧光定量法检测AT1R mRNA及转化生长因子β1(TGFβ1)mRNA的含量。结果氯沙坦组小鼠肝组织病理学改善明显;与感染组相比,氯沙坦组小鼠血清中HA含量降低,肝组织αSMA、TIMP-1的水平和AT1R mRNA、TGF1βmRNA的相对量均显著下降(P<0.05)。结论研究结果显示,氯沙坦改善肝组织病理学改变,降低血清中HA含量,与其能减少αSMA、TIMP-1的表达,下调AT1R mRNA、TGFβ1mRNA的水平有关。OBJECTIVE To study the part of the relevant mechanisms of losartan on hepatic fibrosis of mice infected with Schistosoma japonicum.METHODS Thirty-six kunming mice were randomly divided into three groups： the normal control group,the infectious control group and the test（losartan） group.Schistosomal liver fibrosis model was established by challenge with 35 cercariae S.japonicum.The test group was treated by losartan for 6 weeks.After 6 weeks of treatment,liver samples of the mice were obtained for observing the histopathological changes,the radioimmunoassay was used to detect the serum（HA） in hepatic fibrosis,the（SP） immunohistochemistry and image pro-plus 5.0 were applied to detect the expression of α（SMA）,（TIMP）-1,and the real-time PCR was applied to detect the quantity of AT1R mRNA and transforming growth factor β1（TGFβ1） mRNA.RESULTS Compared with infectious control group,the liver histopathology was improved significantly.Losartan could significantly reduce（HA）;and the levels of αSMA and TIMP-1 were degraded（P0.05）.The quantity of AT1R mRNA and TGFβ1 mRNA in losartan group was lower than that in infectious control group（P0.05）.CONCLUSIONS The losartan group can obviously improve liver histopathology,reduce serum HA.The possible reason is to markedly inhibit the expression of αSMA,TIMP-1,AT1R mRNA and TGFβ1 mRNA.

关 键 词：肝纤维化 日本血吸虫 氯沙坦 αSMA TIMP-1 AT1R MRNA TGFΒ1 MRNA 

分 类 号：R978.1[医药卫生—药品]