海洛因依赖对大鼠睾丸表皮生长因子、β-内啡肽阳性细胞及血清卵泡刺激素的影响  

Effects of heroin dependence on rat's epidermal growth factor,β-endrophin positive cells in testis and serum follicle-stimulating hormone

作　　者：郭斌[1] 梁文妹[1] 

机构地区：[1]贵阳医学院组织学与胚胎学教研室,贵阳550004

出　　处：《解剖学报》2010年第4期545-549,共5页Acta Anatomica Sinica

基　　金：贵州省优秀科技人才省长基金资助项目[(2005)14号];贵州省高层次人才基金资助项目[(2007)26号]

摘　　要：目的探讨海洛因依赖对大鼠睾丸表皮生长因子(EGF)、β-内啡肽(β-EP)阳性细胞的影响和血清卵泡刺激素(FSH)的变化。方法成年雄性SD大鼠55只,随机分为正常对照组、盐水对照组及海洛因依赖组。皮下注射海洛因建立海洛因依赖大鼠模型,分别于模型建立的第10、17、24、31、38天取睾丸组织。采用免疫组织化学SABC法、细胞计数、图像分析方法及放射免疫测定技术进行研究。结果 1.与正常及盐水对照组比较,海洛因依赖组大鼠睾丸EGF阳性细胞免疫染色强度有所增强,平均灰度值明显低于正常及盐水对照组,但细胞数量有所减少(P<0.05)。2.海洛因依赖组大鼠睾丸β-EP阳性细胞数量有所减少(P<0.05),免疫染色强度较正常对照组和盐水对照组有所减弱,平均灰度值逐渐增高(P<0.05)。3.放射免疫测定结果表明,血清FSH在第10天水平较低,第17天有所增高。结论海洛因依赖期间,大鼠睾丸EGF阳性细胞的分泌功能增强,β-EP阳性细胞的分泌功能减弱,两者细胞数量均有所减少,血清FSH分泌量有所改变。提示,睾丸内分泌细胞在海洛因依赖期间可能参与了机体神经内分泌的调节过程。Objective To explore the effects of heroin dependence on the epidermal growth factor （EGF）, β- endrophin（β-EP） positive cells in the testis of rats and the changes of the serum levels of follicle-stimulating hormone （FSH）, comprehend the possible functions of EGF, β-EP and FSH during heroin dependence. Methods Immunohistochemical SABC method, radioimmunoassay （RIA） , counting the number of cells and image analysis were used in the studies. Results 1. Compared with the normal control group （NCG） and saline control group （SCG） , the number of EGF positive ceils decreased （P 〈 0.05） , the intensity of staining stepped up gradually with the passage of time during dependence. The result of image analysis showed that the mean gray degree of the EGF positive cells decreased. 2. The number of β-EP positive cells in heroin dependence group（HDG） were less than that in NCG and SCG （P 〈 0. 05 ）. The immunohistochemieal staining intensity of β-EP positive cells in testis decreased as compared with SCG and NCG and the means of gray degree increased gradually during heroin dependence （ P 〈 0. 05 ）. 3. The RIA result showed that the level of FSH was the lowest on the lOth day and then elevated as compared with SCG and NCG on the 17th clay during heroin dependence. Conclusion During heroin dependence, the secretion function of the testis EGF positive cells was enhanced while the function the β-EP positive cells was weakened. The number of EGF, β-EP positive cells decreased markedly in testis. The secretory volume of FSH changes. The above findings indicated that testis may be involved in the regulations process of nero-endocrine during herion dependence.

关 键 词：表皮生长因子 Β-内啡肽 卵泡刺激素 海洛因依赖 睾丸 免疫组织化学 大鼠 

分 类 号：R329.1[医药卫生—人体解剖和组织胚胎学]