磺胺甲基噁唑和恩诺沙星在日本囊对虾体内的药代动力学研究  

Pharmacokinetics of Sulfamethoxazole(SMZ ) and Enrofloxac-in (ERFX) in shrimp Marsupenaeus japonicus

作　　者：宋维彦[1,2] 苏永全[1] 潘滢[1] 曾凡荣[1] 游欣欣[1] 

机构地区：[1]厦门大学海洋与环境学院,福建厦门361005 [2]日照职业技术学院水产学院,山东日照276826

出　　处：《海洋科学》2010年第7期22-27,共6页Marine Sciences

基　　金：山东省高等学校优秀青年教师国内访问学者项目和公益性行业(农业)科研资助专项(200803012)

摘　　要：为了研究磺胺甲基噁唑(Sulfamethoxazole,简称SMZ)和恩诺沙星(Enrofloxacin,简称ERFX)在日本囊对虾(Marsupenaeus japonicus)体内的药物代谢动力学特点,在水温21℃±0.5℃,盐度29.91的条件下,应用萃取、液相测定和反相高效液相色谱法(RP-HPLC)对药物平均回收率、主要动力学参数及在3种组织中的半衰期进行了测定,检测数据经药物代谢动力学软件3p97进行研究。结果表明,SMZ和ERFX在对虾肝脏、肌肉和血淋巴的平均回收率分别为90.09%,88.92%,84.84%和89.14%,86.04%,91.77%。药物代谢动力学分析表明,SMZ单次腹部肌肉注射日本囊对虾,其肝脏药时数据符合二室模型,肌肉和血淋巴药时数据符合一室模型,半衰期为19.1284,4.5799,9.1855 h。ERFX单次腹部肌肉注射日本囊对虾,其肌肉药时数据符合二室模型,肝脏和血淋巴药时数据符合一室模型。半衰期分别为15.16,16.83,17.19 h。通过两种药物的代谢特征比较分析表明,SMZ在肌肉中吸收较ERFX快,ERFX在血淋巴和肝脏中吸收较SMZ快。根据SMZ和ERFX在21℃±0.5℃的条件下的主要动力学参数及3种组织内消除半衰期,建议SMZ在日本囊对虾的休药期不少于10 d,ERFX的休药期不少于12 d。In order to study the pharmacokinetics of sulfamethoxazole（SMZ）and enrofloxacin （ERFX）in the hemolymph, muscle and liver of Marsupenaeus japonicus, average recoveries, main kinetic parameters and elimination half-times of SMZ and ERFX in three tissues, were measured by HPLC. The average recoveries of SMZ or ERFX in the liver, muscle and hemolymph, respectively, were 90.09%, 88.92%, and 84.84%, or 89.14%, 86.04%, and 91.77%.After a single abdominal intramuscular injection of SMZ, the pharmacokinetics of muscles could be well described by a two-department model, but those of liver and hemolymph were in line with a one- department model; the elimination half-lives were 19.1284, 4.5799, and 9.1855 h, respectively. After a single abdominal intramuscular injection of ERFX, the pharmacokinetics of muscles were in line with the two-department model, but those of liver and hemolymph were in line with the one-department model; the elimination half-lives were 15.16, 16.83, and 17.19 h, respectively. Based on the metabolic characteristics, ERFX were absorbed into haemolymph and liver faster than SMZ, but SMZ was absorbed into muscles faster than ERFX. According to the elimination half-lives of SMZ and ERFX in hemolymph, muscle and liver , we suggested that at 21℃±0.5℃ the withdraw time of SMZ was no less than 10 d, and that of ERFX was no less than 12 d .

关 键 词：日本囊对虾(Marsupenaeus japonicus) 磺胺甲基唑 恩诺沙星 药代动力学 

分 类 号：S948[农业科学—水产养殖]