甲真菌病患者多部位红色毛癣菌感染分离株DNA同源性分析  

DNA sequence homology analysis of Trichophyton rubrurn isolates from multiple body sites of patients with onychomycosis

作　　者：李昌吉[1] 骆志成[1] 

机构地区：[1]兰州大学第二医院皮肤科,730030

出　　处：《中华皮肤科杂志》2010年第8期542-545,共4页Chinese Journal of Dermatology

摘　　要：目的 探讨甲真菌病患者多部位红色毛癣菌感染分离株基因型的差异.方法 采用PCR扩增红色毛癣菌rDNA非转录间隔区（NTS）中TRS1片段,并用随机引物OPAA11随机扩增DNA,检测基因多态性.比较同一患者不同感染部位分离菌株的基因型差异.结果 共分离30株红色毛癣菌,按照PCR扩增TRS1区指纹图分为5型,随机引物OPAA11扩增指纹图分为11型,基因型分布与感染部位关系不大.在10例受试患者中,PCR扩增TRS1区显示,7例不同感染部位分离得到的菌株基因型有差异;随机引物OPAA11扩增显示,8例不同感染部位分离得到的菌株基因型有差异.结论 甲真菌病患者不同部位红色毛癣菌感染可能为不同菌株引起,提示部分甲真菌病患者不同部位皮肤癣菌感染可能存在不同菌株的感染.Objective To profile genotypes of Trichophyton rubrum isolates from different body sites in patients with onychomycosis. Methods DNA was extracted from 30 T. nibium isolates from 10 patients with onychomycosis, and subjected to PCR with tandemly repetitive subelement 1 （TRS1 ）-specific primer to analyze the number of repetitive elements in the non-transcribed spacer （NTS） region of ribosomal DNA gene, and to random primer amplification with the random primer OPAA11. The genotype variety was evaluated for T. rubrum isolates from different body sites of patients with onychomycosis. Results All the strains were classified into 5 genotypes based on the copy number of TRS1, and into 11 genotypes by RAPD analysis. The genotypes of T. rubrum seemed unrelated to sites of infection. Genotype diversity was observed among T. rubrum strains from different body sites of the same host in 7 out of the 10 cases as shown by amplification of TRS1 region, in 8 out of the 10 cases as demonstrated by RAPD analysis. Conclusion A single host with onychomycosis could harbor multiple genotypes of T. rubrum at different body sites, suggesting external sources of infection rather than infection from a different site in the same individual.

关 键 词：发癣菌属 真菌分型技术 随机扩增多态DNA技术 癣 

分 类 号：R756[医药卫生—皮肤病学与性病学]