色谱累加进样分离实验条件的研究  

Studies on Experimental Conditions of Chromatographic Separation with Multiple Injection

作　　者：张维冰[1,2] 许国旺[1,2] 李瑞江[1,2] 张玉奎 

机构地区：[1]中国科学院大连化学物理研究所 [2]国家色谱研究分析中心

出　　处：《分析测试学报》1999年第2期21-24,共4页Journal of Instrumental Analysis

摘　　要：在采用反相液相色谱或亲和色谱完成蛋白质等大分子分离时，根据溶质保留值随溶剂梯度变化曲线上突变点的差别，可以通过累加进样分离法进行样品制备或直接柱内富集分析，但这一方法并非在任意条件下、对任何样品都适用。该文根据不同形式的保留值方程从理论上探讨了样品保留值与进样时间差、梯度洗脱速率等实验条件之间的关系；结果表明：两次进样的出峰时间差与进样时间间隔成正比关系，也与其在等度情况下的容量因子有关。样品中的两种组分在间隔进样时的流出时间差主要由两组分的容量因子决定，当样品中存在两种以上保留性能相近组分时，若保证指定的分离度，进样时间间隔存在一极大值，超出该范围。In reversed _ phase LC and affinity chromatography some macromolecules, e.g.proteins can be separated and purified in a column with multiple injection, however the method is not often applied for different experimental conditions and samples. The relationship of retention of components in sample with chromatographic conditions, such as interval of injection, gradient elution rate was theoratically discussed based on different retention equations. The results show that the capacity factor of two solutes in a sample and the interval between two injections are the main factors effecting the separation. The resolution of two components in a sample with multiple injection depends on the retention times, dead _ time, and the changing rate of the composition of mobile phase. There is a threshold of the interval between two injections for a desired resolution in the isocratic elution.

关 键 词：色谱 累加进样 分离 进样 

分 类 号：O657.706[理学—分析化学]