免疫吸附治疗用ProteinA切流膜色谱柱的研究  

Protein A Tangential Flow Membrane Chromatographic Column Used for Immunoadsorption Therapy

作　　者：贾凌云[1] 杨利[1] 邹汉法[1] 张玉奎[1] 赵久阳[2] 范成明[3] 沙丽艳 

机构地区：[1]中国科学院大连化学物理研究所色谱开放实验室 [2]大连医科大学附属二院肾内科,大连116023 [3]大连医科大学附属二院风湿免疫科,大连116023

出　　处：《色谱》1999年第2期107-111,共5页Chinese Journal of Chromatography

基　　金：国家科技部"九五"攻关项目

摘　　要：切流膜色谱柱是免疫吸附治疗中实现全血灌流的一种新的模式。实验研究表明，切流膜色谱柱的结构和血液流动形式对血细胞的损害很小。分别考察了水、血浆、血液等不同流体流速与切流膜色谱柱柱压降之间的关系，柱压降随着流体流速和粘度的升高而增高，血液流速为120mL／min时，柱压降达到93kPa；考察了ProteinA切流膜色谱柱对人血浆中免疫球蛋白IgG的吸附能力，切流膜色谱柱ProteinA健合量为139mg（6mgProteinA／g干介质），血浆在柱中循环1h可吸附IgG553mg（23．8mgIgG／g平介质），而血液在柱中循环1h,可吸附IgG499.4mm（21.5msIgG／g卡介质），循环时间对IgG吸附量影响较大，循环速度对IgG吸附量影响不大。对狗的体外临床血液灌流实验表明，切流膜色谱柱的血液相容性良好。Tangential flow membrane chromatographic column is a new model of immunoadsorption therapy from blood. The results showed that the structure of the column and the flowing channel had a little damages for blood cells. The relation of flow-rate and backpressure with water, plasma and blood individually as the fluid flowing through the column has been studied. It was observed that backpressures increased with fluidviscosity, when flow-rate of blood was 120 mL/min and the backpressure reached 0. 093 MPa; The adsorption capacity of protein A of the column for IgG from human plasma has been measured. When plasma and blood individually circulated through the column for 1 h, the column with 139 mg protein A immobilized on the matrix (6 mg protein A/g dry matrix) adsorbed 553 mg IgG (23. 8 mg IgG/g dry matrix) from human plasma and 499. 4 mg IgG (21. 5 mg IgG/g dry matrix) from human blood respectively. The circulation time had a great influence on IgG adsorption capacity ; but the circulation rate had a little influence on IgG adsorption capacity. The result of extracoporeal blood per fusion for dog showed that the Protein A column has good blood biocompatibility.

关 键 词：切流 膜色谱柱 免疫吸附 proteinA IGG 全血灌流 

分 类 号：R459.5[医药卫生—治疗学]