高效液相色谱法测定小鼠脑组织单胺递质及其相关化合物  

Detennination of Monoamine Transmitters and Related Compounds in Mouse Brain by High Performance Liquid Chromatography With Fluorescence Detection

作　　者：陈本美[1] 夏令伟[1] 梁绍先[1] 邓富良[1] 黄新华[2] 谭小艳[3] 

机构地区：[1]湖南医科大学分析测试中心,长沙410078 [2]湖南医科大学设备处 [3]湖南医科大学卫生学教研室

出　　处：《分析化学》1999年第3期249-253,共5页Chinese Journal of Analytical Chemistry

摘　　要：采用苯基柱等度洗脱分离,自然荧光检测法,直接测定小鼠脑组织匀浆中单胺递质及其相关化合物.优化出了最佳色谱条件,流动相为20mmol/L柠檬酸三钠(pH4.50)-甲醇(95:5,V/V);柱温为35℃.该方法能同时检测8种化合物.最低检测限为1.0～2.5μg/L;回收率均在93%以上;线性范围为1.0μg/L～1.0mg/L.A rapid and simple isocratic elution chromatagraphic method for the determination of monoamine transmitters and related compounds using their native fluorescence by high performance liquid chromatography was developed. CLC-phenyl column was selected as separation column at 35℃, and 20 mmol/L of trisodium citrate(pH 4.50)-methanol (95:5, V/V) was isocratic eluted at flow rate of 1.0 mL/min. Eight compounds were simultaneously separated and determined. The recoveries of all compounds were above 93% , the detection limit was 1.0 μg/L- 2.5 μg/L and the linear range was 1.0 μg/L- 10 mg/L for compounds.

关 键 词：单胺递质 脑组织 HPLC 测定 儿茶酚胺 肾上腺素 

分 类 号：Q42[生物学—神经生物学] Q577.03[生物学—生理学]