肉苁蓉及其醇提物的傅里叶变换红外光谱研究  

Study on Cistanche deserticola and their ethanol extracts by Fourier transform infrared spectroscopy

作　　者：刘友刚[1,2] 王威[2] 徐荣[1] 孙素琴[3] 陈君[1] 刘同宁 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所濒危药材繁育国家工程实验室,北京100193 [2]天津中医药大学基础医学院,天津300193 [3]清华大学化学系,北京100084 [4]宁夏永宁本草苁蓉种植基地,宁夏银川750010

出　　处：《中国医院药学杂志》2010年第15期1257-1260,共4页Chinese Journal of Hospital Pharmacy

基　　金：国家自然科学基金资助项目(编号:30772727);国家科技支撑计划项目(编号:2006BAI06A13)

摘　　要：目的:建立红外光谱法评价肉苁蓉原药材及其醇提物中苯乙醇苷类物质的含量。方法:肉苁蓉属2种常用药材肉苁蓉、管花肉苁蓉采用95%乙醇进行提取,采用压片法分别测定原药材及提取物400～4000cm范围内的红外吸收光谱。结果:(1)管花肉苁蓉中与松果菊苷和毛蕊花糖苷有关的吸收峰1692,1631,1604,1516,1447,1158,812cm-1处出现几率明显高于肉苁蓉,提示管花肉苁蓉中苯乙醇苷类物质的含量较高。(2)肉苁蓉药材经过提取后,松果菊苷、毛蕊花糖苷等有效成分的含量明显增加,松果菊苷的最强吸收峰1067cm-1峰在管花肉苁蓉醇提物中出现。同时,肉苁蓉醇提物在1630,1521,1156,981cm-1处出现吸收峰,与原药材相比,肉苁蓉醇提物中的苯乙醇苷类物质得到了有效富集。而相应的二阶导数谱进一步验证了管花肉苁蓉中苯乙醇苷类物质较荒漠肉苁蓉高。结论:红外光谱法结合二阶导数谱能够快速、有效和简便的提供复杂混合物中主要成分的结构信息和量的信息,为中药材的质量控制和定量分析提供了理论依据。OBJECTIVE Fourier transform infrared （FTIR） spectroscopy associated with second derivative infrared spectros copy was applied to study on the 2 herbal sources of Cistanche deserticola and their alcohol extracts. METHODS Powder of different species and their alcohol extracts were directly compressed and determined by Fourier transformation intermediate infrared spectrum. RESULTS The IR spectra showed that： （1）The bonds related to echinacoside, acteoside of C. tubulosa： 1692, 1631, 1604, 1516, 1447, 1158, 812 cm ^-1 appeared more frequnently than C. deserticola, indicating that contents of echinacoside and acteoside in C. tubulosa are much higher. （2） In the ethanol extracts, contents of echinacoside and acteoside increased. The typical and strongest absorption band in IR spectrum of echinacoside is at 1067 cm ^-1 which exists in the IR spectrum of C. tubulosa extract. Moreover, it can be seen that the echinacoside and acteoside in ethanol extracts are more ap parent. In addition, the corresponding secondary derivative spectra further confirm that the contents of echinacoside and acteoside are higher in Cistanche tubulosa. CONCLUSION FT-IR associated with second derivative infrared spectroscopy is a quick and effective analytical technique, which could provide valuable information about chemical constituents of complicated mixture system for monitoring the inherent qualities of traditional Chinese medicine and its corresponding extract.

关 键 词：傅里叶变换红外光谱 肉苁蓉 管花肉苁蓉 醇提物 

分 类 号：R927.2[医药卫生—药学]