不同时段应用他克莫司纳米微球缓释颗粒促进同种异体神经移植再生的实验研究  

Effects of controlled release of FK506 by P （DLLA.co-TMC） microspheres at different intervals on regeneration after allogenic nerve graft in a rodent model

作　　者：柯于海[1] 张振伟[1] 冯登殿[1] 廖坚文[1] 李征[1] 庄加川[1] 

机构地区：[1]广州医学院附属深圳沙井医院手外科, 深圳518104

出　　处：《中华手外科杂志》2010年第4期230-233,共4页Chinese Journal of Hand Surgery

摘　　要：目的探讨不同时间段使用他克莫司（FK506）纳米微球在同种异体移植后促进神经的再生作用。方法采用单乳化-溶剂挥发法（O／W）制备FK506纳米微球，同种异体移植大鼠胫神经。A组术后立即局部应用FK506纳米微球，B组24h给药，C组3d后给药，D组不给药；于术后第4、8和12周行移植神经大体观察、组织学检查及有髓纤维图像分析、小腿三头肌湿重测定、荧光素逆行标记神经元、双侧胫神经电生理比较。结果FK506纳米微球降解快、吸收好。A、B组神经再生效果相似，都明显要比C、D组好，差异有统计学意义；C、D组之间差异也有统计学意义。结论FK506纳米微球释药速度符合神经损伤后再生规律，局部应用有良好的促进神经再生作用，而且早期（24h内）给药效果更加明显。Objective To study the effect of the application of P（DLLA-co-TMC）/FK506 microspheres at different intervals on peripheral nerve regeneration after tibial nerve allograft. Methods P （DLLA-co- TMC）/FK506 mierespheres were fabricated using an oil-in-water single emulsion and solvent evaporation method. Tibial nerve allograft model was created in mice which were divided into group A, B, C and D. In groups A, B and C were P（DLLA-co-TMC）/FK506 mierospheres were administered locally on the day of surgery, 24 hours postoperatively, and 3 days postoperatively, respectively. Group D was left untreated. On postoperative weeks 4, 8 and 12, gross observation of the graft, histomorphometrie image analysis, triceps surae wet weight, retrograde tracing and fluorescence microscopy of motor neurons, and eleetrophysiologieal examination of bilateral fibial nerves were carried out. Results P （DLLA-co-TMC）/FKS06 mierespheres degraded fast and were well absorbed. There was a statistically significant improvemem in neuroregenemtion in groups A and B comparing to that in groups C and D. There was also significant difference between groups C and D. Condlusion The releasing kinetics of P（DLLA-co-TMC）/FK506 rnicrospheres matches the principle of nerve regeneration. It has remarkable effects on neuroregeneration, especially when it is locally administered earlier（ within 24 hours）.

关 键 词：移植 周围神经 再生 FK506纳米微球 同种异体 

分 类 号：R651.3[医药卫生—外科学]