大气交通污染物对雄性小鼠生殖系统影响  

Effect of atmospheric traffic pollutants on male reproductive system in mice

作　　者：孙娜[1] 郑铃[1] 王征桦[1] 李旭冰[1] 王章敬[1] 

机构地区：[1]福建医科大学公共卫生学院流行病学与统计学系,福州350004

出　　处：《中国公共卫生》2010年第8期1045-1047,共3页Chinese Journal of Public Health

基　　金：福建省科技厅计划重点项目基金(2007Y0017)

摘　　要：目的探讨暴露于大气交通污染物对雄性小鼠生殖系统影响。方法 15只雄性昆明系小鼠随机分为3组:重度污染暴露组、中度污染暴露组和相对清洁对照组,每组5只,每日现场暴露10 h。小鼠暴露26 d后,称量体重、睾丸重量并计算脏器系数,然后睾丸和附睾经组织切片、苏木素-伊红(HE)染色,在光镜下观察组织病理学变化。结果对照组小鼠平均体重为27.88 g,睾丸重量为0.140 2 g,而重度污染暴露组小鼠平均体重为25.60 g,睾丸重量为0.115 7 g,经检验差异均有统计学意义(P<0.05);与对照组比较,重度污染暴露组小鼠生精细胞层数由正常的5～7层减少到1～2层,中度污染暴露组小鼠生精细胞层数由正常的5～7层减少到3～4层,差异均有统计学意义(P<0.01);与对照组比较,中度、重度污染暴露组精子数量均明显减少且差异有统计学意义(P<0.01)。结论大气交通污染物对雄性小鼠生殖系统会造成一定损伤。Objective To investigate the effect of atmospheric traffic pollutants on male reproductive system in mice.Methods Fifteen Kunming male mice were randomly divided into three groups（heavy pollution,moderate pollution group and non-pollution group）.The mice in pollution groups were exposed to traffic pollution at field for 10 hours every day.Twenty-six days after the exposure,body and testis weight of the mice were weighted and the organ weight ratio was calculated.The testis and epididymis were collected and dyed with HE staining for observation of pathological changes under light microscope.Results The average body weight,testicular weight of the mice in heavy pollution group were significantly lower than that of control group（25.60 vs 27.88 g,0.115 7 vs 0.140 2g;P0.05）.Compared to the control group,germ cell layers of the mice exposed to heavy pollution decreased from 5-7 layers to 1-2 layers,and that of the moderate pollution decreased from 5-7 to 3-4 layers with significant differences（P0.01）.Compared with the mice in control and moderate pollution groups,the number of sperm in mice exposed to heavy pollution were significantly reduced with significant diffences（P0.01）.Conclusion The atmospheric transport pollutants have damage effects on male reproductive system in mice.

关 键 词：大气交通污染物 睾丸 附睾 精子 

分 类 号：R12[医药卫生—环境卫生学]