南方某市儿童尿中1-羟基芘水平检测  

Determination of PAHs exposure among unban children in south China

作　　者：岳强[1,2] 范瑞芳[3] 于志强[1] 盛国英[1] 傅家谟[1] 

机构地区：[1]中国科学院广州地球化学研究所有机地球化学国家重点实验室,广东广州510640 [2]韶关学院英东生物工程学院 [3]华南师范大学生命科学学院

出　　处：《中国公共卫生》2010年第8期1078-1079,共2页Chinese Journal of Public Health

基　　金：中国科学院知识创新工程重要方向项目(KZCX2-YW-403)

摘　　要：目的探讨中国南方某市<6岁儿童尿液中1-羟基芘的暴露水平。方法采集某幼儿园80名4～6岁儿童晨尿,尿液经过酶解、固相萃取(SPE)富集、净化,采用高效液相色谱-荧光检测器检测1-羟基芘含量。结果在所有样品中均检测出1-羟基芘,浓度范围0.10～1.64μmol/mol肌酐,浓度中位数为0.38μmol/mol肌酐;儿童尿液中1-羟基芘浓度水平不仅均高于国内背景人群,也高于国外儿童,其中>75%儿童尿液中1-羟基芘的浓度高于一般人群暴露限值;该人群体内多环芳烃(PAHs)来源和暴露途径复杂。结论南方某市<6岁儿童可能处于较高PAHs暴露风险。Objective To investigate concentrations of 1-hydroxy pyrene（1-OHP） in the urine of childrens younger than six years from a city of south China.Methods Eighty urine samples were collected from the children in a kindergartens.After enzymic hydrolysis,the urine samples were preconcentrated and cleaned up by solid phase extraction.Then the urinary 1-OHP was determined by high performance liquid chromatography with fluorescence detection.Results The 1-OHP was detected in all urinary samples.The concentration of 1-OHP ranged from 0.10 to 1.64 μmol/mol creatinine,and the concentration median was 0.38 μmol/mol creatinine.The concentration of urinary 1-OHP was not only higher than those of the population in China,but also higher than that of those of the similar children in other countries.Over 75% of urine samples showed higher I-OHP levels than that of biologic exposure limits（BEL）.Conclusion The children in the kindergarten of the city might have a higher risk of PAHs exposure.

关 键 词：儿童 多环芳烃 1-羟基芘 高效液相色谱 

分 类 号：R181.3[医药卫生—流行病学]