温度与饲粮维生素C水平对后备蛋鸭血清免疫和抗氧化指标的影响  

Effects of Temperature and Dietary Vitamin C Levels on Serum Immune and Antioxidative Indices in Reserved Laying Ducks

作　　者：李士平[1] 宋树豪[1] 范成浩[1] 王安[1] 

机构地区：[1]东北农业大学动物营养研究所,哈尔滨150030

出　　处：《动物营养学报》2010年第4期923-928,共6页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金资助项目(30972111)

摘　　要：本试验旨在研究温度与饲粮维生素C水平蛋鸭的血清免疫和抗氧化指标的影响。选取12周龄金定鸭216只,温度设低温(2±1)℃和适温(18±1)℃2个水平;饲粮中的维生素C添加水平分别为0、200和800mg/kg。试验共6个处理,每个处理6个重复,每个重复6只试验鸭。试验期为6周。试验结果表明:1)低温使血清维生素C浓度显著降低了27.78%(P<0.05),对氧化氢酶(CAT)、总抗氧化能力(T-AOC)、丙二醛(MDA)、白介素2(IL-2)、免疫球蛋白(IgG、IgA、IgM)、生长激素(GH)、胰岛素(Ins)、胰岛素样生长因子(IGF-Ⅰ)、瘦素(Lep)、皮质醇(Cort)、三碘甲状腺原氨酸(T3)和甲状腺素(T4)的含量影响显著(P<0.05);2)饲粮中添加200mg/kg维生素C时,血清维生素C、IGF-Ⅰ、Lep水平显著升高(P<0.05),Cort浓度显著降低(P<0.05);3)温度和维生素C的交互作用对Cort的含量的影响极显著(P<0.01),对IGF-Ⅰ、Lep、IgG和IgA的含量影响显著(P<0.05),对GH、Ins、维生素C、T3、T4及各个抗氧化指标的影响没达到显著水平(P>0.05)。结果提示,低温影响后备蛋鸭血清维生素C的浓度,但饲粮中添加200mg/kg维生素C时,可提高在低温条件下的后备蛋鸭血液维生素C的水平、提高血液中Lep和IGF-Ⅰ的浓度、增强机体抗氧化和抗寒冷应激能力。This study was designed to evaluate the effects of temperature and dietary vitamin C levels on serum immune and antioxidative indices in reserved laying ducks.Two hundred and sixteen Jinding ducks（12 weeks old）were chosen and randomly divided into 6 treatments which were exposed to the environment at（2±1）℃ and（18±1）℃,dietary vitamin C was added at 0,200 and 800 mg/kg,respectively.The trial lasted for six weeks.The results showed as follows：1）the content of vitamin C in serum was significantly decreased by 27.78%（P0.05）,while there were significant effects on the contents of CAT,T-AOC,MDA,IL-2,IgG,IgA,IgM,GH,Ins,IGF-Ⅰ,leptin,Cort,T3 and T4（P0.05）in the situation of cold stress;2）the levels of serum vitamin C,IGF-Ⅰ and leptin were increased（P0.05）and Cort level was significantly decreased（P0.05）by 200 mg/kg vitamin C supplementation;3）the interaction of temperature and vitamin C significantly affected the contents of Cort（P0.01）and IGF-Ⅰ,leptin,IgG and IgA（P0.05）,there were no significant effects on GH,Ins,vitamin C,T3,T4 and antioxidant indices（P0.05）.It was concluded that the ability of vitamin C biosynthesis was influenced by low temperature,the contents of serum vitamin C,leptin,IGF-I and the ability of antioxidant and anti-cold stress were improved when adding 200 mg/kg vitamin C.

关 键 词：温度 维生素C 后备蛋鸭 生理反应 

分 类 号：S834[农业科学—畜牧学]