溃疡平对胃溃疡大鼠胃黏膜组织中VEGF和Flt-1 mRNA表达的影响  

Influences of Kuiyangping Decoction on expressions of VEGF and Flt-1-mRNA in gastric mucosa in rats with gastric ulcer

作　　者：朱立革[1,2] 曹军[3] 赵红晔[1] 周丽[1] 王滨[1] 

机构地区：[1]齐齐哈尔医学院基础医学部,黑龙江161006 [2]黑龙江中医药大学研究生学院 [3]中国中医科学院中药研究所

出　　处：《北京中医药大学学报》2010年第7期476-479,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：黑龙江省研究生创新科研基金资助项目(No.YJSCX2009-239HLJ)

摘　　要：目的观察溃疡平对胃溃疡大鼠胃黏膜组织血管内皮生长因子(VEGF)和血管内皮生长因子受体-1(Flt-1)mRNA表达的影响,探讨溃疡平促进大鼠胃溃疡愈合的机制。方法采用改良Okabe法制备慢性乙酸胃溃疡模型,随机分为:正常对照组,模型对照组,溃疡平高、中、低剂量(5.6、2.8、1.4g/kg)组,盐酸雷尼替丁(28.3mg/kg)组。给药治疗14d,采用实时荧光定量PCR法检测胃黏膜组织VEGF和Flt-1mRNA表达。结果与模型对照组相比,溃疡平中、高剂量组的VEGF2-ΔΔCT值明显增高,表明溃疡平中、高剂量组的VEGFmRNA表达上调,有统计学意义(P分别<0.05和<0.001);溃疡平高剂量组的Flt-12-ΔΔCT值明显增高,表明溃疡平高剂量组的Flt-1mRNA表达上调,有统计学意义(P<0.001)。结论溃疡平可促进胃溃疡大鼠胃黏膜组织VEGF和Flt-1mRNA表达,这可能是其促进溃疡愈合,提高溃疡愈合质量,减少溃疡复发的分子学机制之一。Objective To observe the influences of Kuiyangping Decoction（KD） on the expressions of mRNA of vascular endothelial growth factor（VEGF-mRNA） and VEGF receptor（Flt-1-mRNA） in gastric mucosa in rats with gastric ulcer（GU） ,and to discuss the mechanism.Method The model of chronic acetic-related GU was established by applying modified Okabe method,and then all rats were randomly divided into the normal group,model group,high-dose,mid-dose and low-dose KD groups（5.6,2.8,1.4 g/kg） and ranitidine hydrochloride group（28.3 mg/kg） .All groups were treated for 14 days.The expressions of VEGF-mRNA and Flt-1-mRNA were detected by using RT-PCR.Result In the mid-dose and high-dose KD groups,compared with the model group,the 2-ΔΔC T value of VEGF increased significantly,which indicated that the expression of VEGF-mRNA was regulated upwards with a statistical significance（P0.05,P0.001 respectively） .In the high-dose KD group the 2-ΔΔC T value of Flt-1 increased,which indicated that the expression of Flt-1-mRNA was regulated upwards with a statistical significance（P0.001） .Conclusion KD can increase the expressions of VEGF-mRNA andFlt-1-mRNA in gastric mucosa in rats with GU,which may be one of molecular bases of improving the healing of GU and healing quality,and reducing recurrence of GU.

关 键 词：溃疡平 胃溃疡 血管内皮生长因子 血管内皮生长因子受体-1 实时荧光定量PCR 大鼠 

分 类 号：R285.5[医药卫生—中药学]